Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD133 antibody [EPR20980-45] - BSA and Azide free (ab226486)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20980-45] to CD133 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CD133 antibody [EPR20980-45] - BSA and Azide free
    See all CD133 primary antibodies

  • Description

    Rabbit monoclonal [EPR20980-45] to CD133 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human pancreas tissue. WB: HAP1 and Caco-2 cell lysates.

  • General notes

    Ab226486 is the carrier-free version of ab226355. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab226486 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CD133 antibody [EPR20980-45] - BSA and Azide free (ab226486)
    Western blot - Anti-CD133 antibody [EPR20980-45] - BSA and Azide free (ab226486)
    All lanes : Anti-CD133 antibody [EPR20980-45] (ab226355) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate at 40 µg
    Lane 2 : PROM1 knockout HAP1 cell lysate at 40 µg
    Lane 3 : Caco-2 cell lysate at 20 µg
    Lane 4 : HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    ab226355 was shown to react with CD133 in wild-type HAP1 cells in western blot. Loss of signal was observed when PROM1 knockout sample was used. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab226355 overnight at 4°C at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with ECL reagent and imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-45] - BSA and Azide free (ab226486)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-45] - BSA and Azide free (ab226486)

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling CD133 with ab226355 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP), ready to use. Apical/endoluminal staining on ducts of human pancreatic cancer (PMID: 18261235) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP), ready to use.
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab226355).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-45] - BSA and Azide free (ab226486)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR20980-45] - BSA and Azide free (ab226486)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD133 with ab226355 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP), ready to use. Apical/endoluminal staining on ducts of human pancreas, and negative on Langerhans cells of the islets (PMID: 18261235) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP), ready to use.
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab226355).

  • Anti-CD133 antibody [EPR20980-45] - BSA and Azide free (ab226486)
    Anti-CD133 antibody [EPR20980-45] - BSA and Azide free (ab226486)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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