Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1302] to Musashi 1 / Msi1 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Chicken, Human, Quail

Overview

  • Product name

    Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free
    See all Musashi 1 / Msi1 primary antibodies

  • Description

    Rabbit monoclonal [EP1302] to Musashi 1 / Msi1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Chicken, Human, Quail

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human lung carcinoma tissue. Flow Cyt: SH-SY5Y cells. ICC: SH-SY5Y and Neuro-2a cells.

  • General notes

    Ab221797 is the carrier-free version of ab52865. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab221797 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
    Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
    All lanes : Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : MSI1 knockout HAP1 whole cell lysate
    Lane 3 : SH-SY5Y whole cell lysate

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab52865 observed at 39 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab52865 was shown to specifically react with Musashi 1 / Msi1 in wild-type HAP1 cells as signal was lost in MSI1 knockout cells. Wild-type and MSI1 knockout samples were subjected to SDS-PAGE. Ab52865 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52865).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Mushashi 1/ Msi1 with Purified ab52865 at 1:50 dilution (17.7 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52865).

  • Flow Cytometry - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
    Flow Cytometry - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)

    Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with purified ab52865 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52865).

  • Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
    Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)

    Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with Purified ab52865 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200. Ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52865).

  • Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
    Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)

    Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) labelling Musashi 1 with purified ab52865 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised by 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52865).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797) This image is courtesy of an Abreview submitted by Carl Hobbs.

    Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Musashi 1 / Msi1 antibody [EP1302] (unpurified ab52865) on Quail Tissue sections (embryo d5/6 Brain stem T/S). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody unpurified ab52865 incubated at 1/300 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52865).

  • Flow Cytometry - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
    Flow Cytometry - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797) This image is courtesy of an Abreview submitted by Jennifer Moore.

    Flow Cyt image of Musashi1 (ab52865)using Accutase digested single cell suspension of hESC. the cells were fixed adn permeabilized. The cells were incubated with unpurified ab52865 (1/20 using Prem/wash solution) for 30 mins at 23°C.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52865).

  • Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
    Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)

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