Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Musashi 1 / Msi1 antibody (ab21628)

Key features and details

  • Rabbit polyclonal to Musashi 1 / Msi1
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Musashi 1 / Msi1 antibody
    See all Musashi 1 / Msi1 primary antibodies

  • Description

    Rabbit polyclonal to Musashi 1 / Msi1

  • Host species

    Rabbit

  • Specificity

    Several customers have found that this antibody gives good results in rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in rat.

  • Tested applications

    Suitable for: ICC, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human Musashi 1/ Msi1 aa 1-100 (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab23870, ab39212)

Properties

Applications

Our Abpromise guarantee covers the use of ab21628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.

    • Tissue specificity

      Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.

    • Sequence similarities

      Belongs to the Musashi family.
      Contains 2 RRM (RNA recognition motif) domains.

    • Domain

      The first RNA recognition motif binds more strongly to RNA compared to the second one.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Msi 1 antibody
      • Msi1 antibody
      • MSI1H_HUMAN antibody
      • Musashi homolog 1 antibody
      • Musashi RNA binding protein 1 antibody
      • Musashi-1 antibody
      • Musashi1 antibody
      • RNA binding protein Musashi homolog 1 antibody
      • RNA-binding protein Musashi homolog 1 antibody
      see all

    Images

    • Western blot - Anti-Musashi 1 / Msi1 antibody (ab21628)
      Western blot - Anti-Musashi 1 / Msi1 antibody (ab21628)
      All lanes : Anti-Musashi 1 / Msi1 antibody (ab21628) at 1 µg/ml

      Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
      Lane 2 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)
      Lane 3 : E14 Mouse Embryo Brain Tissue Lysate
      Lane 4 : E10 Mouse Embryo Brain Tissue Lysate
      Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
      Lane 6 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
      Lane 7 : E14 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
      Lane 8 : E10 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Predicted band size: 39 kDa
      Observed band size: 39 kDa
      Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.



      ab21628 recognized a band corresponding to the expected size of Musashi1/Msi1. The band was blocked using the immunising peptide (ab23870).
    • Immunocytochemistry - Anti-Musashi 1 / Msi1 antibody (ab21628)
      Immunocytochemistry - Anti-Musashi 1 / Msi1 antibody (ab21628)This image is courtesy of an Abreview submitted by Miss Szu-Yu Chen
      ab21628 at 1ug/ml staining human eye (limbal region) by ICC/IF. The cells were paraformaldehyde fixed and blocked with 2% BSA prior to incubation with antibody for 1 hour. A FITC conjugated sheep polyclonal antibody was used as the secondary.

      See Abreview

    • Immunocytochemistry - Anti-Musashi 1 / Msi1 antibody (ab21628)
      Immunocytochemistry - Anti-Musashi 1 / Msi1 antibody (ab21628)
      ICC/IF image of ab21628 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21628, 10µg/ml) overnight at +4°C. The secondary antibody (green) was a96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 10µg/ml.

    References (21)

    Publishing research using ab21628? Please let us know so that we can cite the reference in this datasheet.

    ab21628 has been referenced in 21 publications.

    • Huang C  et al. ERK1/2-Nanog signaling pathway enhances CD44(+) cancer stem-like cell phenotypes and epithelial-to-mesenchymal transition in head and neck squamous cell carcinomas. Cell Death Dis 11:266 (2020). PubMed: 32327629
    • Herrmann A  et al. Integrin a6 signaling induces STAT3-TET3-mediated hydroxymethylation of genes critical for maintenance of glioma stem cells. Oncogene 39:2156-2169 (2020). PubMed: 31819166
    • Yang LY  et al. MicroRNA-331 inhibits development of gastric cancer through targeting musashi1. World J Gastrointest Oncol 11:705-716 (2019). PubMed: 31558975
    • Bhere D  et al. Stem Cells Engineered During Different Stages of Reprogramming Reveal Varying Therapeutic Efficacies. Stem Cells 36:932-942 (2018). PubMed: 29451340
    • Vaine CA  et al. X-linked Dystonia-Parkinsonism patient cells exhibit altered signaling via nuclear factor-kappa B. Neurobiol Dis 100:108-118 (2017). PubMed: 28017799
    View all Publications for this product

    Images

    • Western blot - Anti-Musashi 1 / Msi1 antibody (ab21628)
      Western blot - Anti-Musashi 1 / Msi1 antibody (ab21628)
      All lanes : Anti-Musashi 1 / Msi1 antibody (ab21628) at 1 µg/ml

      Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
      Lane 2 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)
      Lane 3 : E14 Mouse Embryo Brain Tissue Lysate
      Lane 4 : E10 Mouse Embryo Brain Tissue Lysate
      Lane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
      Lane 6 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
      Lane 7 : E14 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml
      Lane 8 : E10 Mouse Embryo Brain Tissue Lysate with Human Musashi 1 / Msi1 peptide (ab23870) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Predicted band size: 39 kDa
      Observed band size: 39 kDa
      Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.



      ab21628 recognized a band corresponding to the expected size of Musashi1/Msi1. The band was blocked using the immunising peptide (ab23870).
    • Immunocytochemistry - Anti-Musashi 1 / Msi1 antibody (ab21628)
      Immunocytochemistry - Anti-Musashi 1 / Msi1 antibody (ab21628) This image is courtesy of an Abreview submitted by Miss Szu-Yu Chen
      ab21628 at 1ug/ml staining human eye (limbal region) by ICC/IF. The cells were paraformaldehyde fixed and blocked with 2% BSA prior to incubation with antibody for 1 hour. A FITC conjugated sheep polyclonal antibody was used as the secondary.

      See Abreview

    • Immunocytochemistry - Anti-Musashi 1 / Msi1 antibody (ab21628)
      Immunocytochemistry - Anti-Musashi 1 / Msi1 antibody (ab21628)
      ICC/IF image of ab21628 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21628, 10µg/ml) overnight at +4°C. The secondary antibody (green) was a96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 10µg/ml.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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