Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19466-105] to PARK7/DJ1
Suitable for: WB, Flow Cyt, ICC/IF, IP
Reacts with: Human

Product name

Anti-PARK7/DJ1 antibody [EPR19466-105]
See all PARK7/DJ1 primary antibodies
Description

Rabbit monoclonal [EPR19466-105] to PARK7/DJ1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB; HeLa, HEK-293T and LNCaP whole cell lysate. Human brain, fetal heart, fetal kidney and fetal spleen lysate. ICC/IF: HeLa and LNCaP cells. Flow Cytometry: HeLa cells. IP: HEK-293T whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19466-105
Isotype

IgG
Research areas

Western blot - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)

All lanes : Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147) at 1/5000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human embryonic kidney) whole cell lysate
Lane 3 : LNCaP (human prostate carcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 20 kDa
Observed band size: 23 kDa
why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)

Immunofluorescent analysis of 100% methanol fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PARK7/DJ1 with ab201147 at 1/100 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weak nuclear staining on HeLa cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)(ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)

Flow cytometric analysis of 4% paraformaldehyde fixed HeLa (human cervix adenocarcinoma) cell line labeling PARK7/DJ1 with ab201147 at 1/120 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)

PARK7/DJ1 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) whole cell lysate with ab201147 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab201147 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293T (human embryonic kidney) whole cell lysate 10 μg (Input).
Lane 2: HEK-293T whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201147 in HEK-293T whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Western blot - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)

All lanes : Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147) at 1/5000 dilution

Lane 1 : Human brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Predicted band size: 20 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)

Immunofluorescent analysis of 100% methanol fixed LNCaP (Human prostate cancer cell line) cells labeling PARK7/DJ1 with ab201147 at 1/100 dilution, followed by AlexaFluo^r®488 Goat Anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weak nuclear staining on LNCaP cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)(ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-PARK7/DJ1 antibody [EPR19466-105] (ab201147)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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