Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)
![Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)](https://www.abcam.com/ps/products/129/ab129819/Images/ab129819-341554-ab129819KOWBGDBIPAL.jpg "Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)")
Key features and details
- Mouse monoclonal [Y2709] to Musashi 1 / Msi1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Musashi 1 / Msi1 antibody [Y2709]
See all Musashi 1 / Msi1 primary antibodies -
Description
Mouse monoclonal [Y2709] to Musashi 1 / Msi1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
corresponding to Musashi 1/ Msi1.
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Positive control
- This antibody gave a positive signal in the following lysates: human brain membrane; E14 rat embryonic brain; SHSY5Y whole cell; 46C whole cell.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
Y2709 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)All lanes : Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819) at 5 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MSI1 knockout HAP1 whole cell lysate
Lane 3 : SH-SY5Y whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 39 kDa
Observed band size: 39 kDaLanes 1 - 3: Merged signal (red and green). Green - ab129819 observed at 39 kDa. Red - loading control, ab52866, observed at 50 kDa.
ab129819 was shown to specifically react with Musashi 1 / Msi1 in wild-type HAP1 cells as signal was lost in MSI1 knockout cells. Wild-type and MSI1 knockout samples were subjected to SDS-PAGE. Ab129819 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 5 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)](https://www.abcam.com/ps/products/129/ab129819/Images/ab129819-144079-anti-musashi-1-msi1-antibody-y2709-western-blot.jpg)
Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)All lanes : Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819) at 5 µg/ml
Lane 1 : Brain (Human) Membrane Lysate - fetal normal tissue (ab29457)
Lane 2 : E14 Rat Embryo Brain Tissue Lysate
Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 4 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
