Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸ 10 µl 147 417 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1302] to Musashi 1 / Msi1
Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Human, Quail

Product name

Anti-Musashi 1 / Msi1 antibody [EP1302]
See all Musashi 1 / Msi1 primary antibodies
Description

Rabbit monoclonal [EP1302] to Musashi 1 / Msi1
Host species

Rabbit
Specificity

Several customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Mouse Human
IHC-P	Human Quail
WB	Chicken Human

See all applications and species data

Immunogen

Synthetic peptide within Human Musashi 1/ Msi1 (N terminal). The exact sequence is proprietary.
(Peptide available as ab178003)
Positive control
- IHC-P: Human lung carcinoma tissue. Flow Cyt: SH-SY5Y cells. ICC: SH-SY5Y and Neuro-2a cells. WB: SH-SY5Y cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1302
Isotype

IgG
Research areas

Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

All lanes : Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MSI1 knockout HAP1 whole cell lysate
Lane 3 : SH-SY5Y whole cell lysate

Predicted band size: 39 kDa
Observed band size: 39 kDa

Lanes 1 - 3: Merged signal (red and green). Green - ab52865 observed at 39 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab52865 was shown to specifically react with Musashi 1 / Msi1 in wild-type HAP1 cells as signal was lost in MSI1 knockout cells. Wild-type and MSI1 knockout samples were subjected to SDS-PAGE. Ab52865 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

All lanes : Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/1000 dilution (purified)

Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lane 2 : UMNSAH/DF-1 (chicken embryo fibroblast) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 39 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with purified ab52865 at 1:80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with Purified ab52865 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200. Ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Mushashi 1/ Msi1 with Purified ab52865 at 1:50 dilution (17.7 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) labelling Musashi 1 with purified ab52865 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised by 0.1% tritonX-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution (unpurified) + SH-SY-5Y cell lysate at 10 µg/ml

Secondary
goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 39 kDa
Observed band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) This image is courtesy of Carl Hobbs, King's College London, United Kingdom

Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Musashi 1 / Msi1 antibody [EP1302] (unpurified ab52865) on Quail Tissue sections (embryo d5/6 Brain stem T/S). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody unpurified ab52865 incubated at 1/300 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).
Flow Cytometry - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) This image is courtesy of an abreview submitted by Jennifer Moore, Stem Cell Research Center, United States

Flow Cyt image of Musashi1 (ab52865)using Accutase digested single cell suspension of hESC (Neural stem cells derived from human embryonic). The cells were fixed adn permeabilized. The cells were incubated with unpurified ab52865 (1/20 using Prem/wash solution) for 30 mins at 23°C.
See Abreview
Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

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