Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)

Key features and details

  • Mouse monoclonal [9-9-3] to SOX2 - BSA and Azide free
  • Suitable for: WB, IHC-Fr, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human, Apteronotus leptorhynchus
  • Isotype: IgG1

Overview

  • Product name

    Anti-SOX2 antibody [9-9-3] - BSA and Azide free
    See all SOX2 primary antibodies

  • Description

    Mouse monoclonal [9-9-3] to SOX2 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Fr, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human, Apteronotus leptorhynchus
    Predicted to work with: Rat, Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Rhesus monkey

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: NCCIT and F9 cells. WB: F9 and PC-3 whole cell lysate. Flow Cyt: F9 cells.

  • General notes

    ab264112 is the PBS only version of ab79351.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)

    This image was produced using the same antibody clone, but in a different formulation, ab79351.

    Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized NCCIT (human pluripotent embryonal carcinoma) cells labeling SOX2 with ab79351 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing showing positive staining on NCCIT cells. Counterstained with ab179504 anti-Tubulin (Rabbit mAb, 1/1000), ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution. Nuclear stained with DAPI.
    The negative controls are as follows:
    -ve control 1: ab79351 at 1/200 dilution, followed by ab150080 at 1/1000 dilution.
    -ve control 2: ab179504 at 1/1000 dilution, followed by ab150113 at 1/1000 dilution.

  • Flow Cytometry - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)
    Flow Cytometry - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)

    This image was produced using the same antibody clone, but in a different formulation, ab79351.

    Overlay histogram showing F9 cells stained with ab79351 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79351, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed F9 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)

    This image was produced using the same antibody clone, but in a different formulation, ab79351.

    Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized F9 (mouse embryonal carcinoma) cells labeling SOX2 with ab79351 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing showing positive staining on F9 cells. Counterstained with ab179504 anti-Tubulin (Rabbit mAb, 1/1000), ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution. Nuclear stained with DAPI.
    The negative controls are as follows:
    -ve control 1: ab79351 at 1/200 dilution, followed by ab150080 at 1/1000 dilution.
    -ve control 2: ab179504 at 1/1000 dilution, followed by ab150113 at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] - BSA and Azide free (ab264112)

    This image was produced using the same antibody clone, but in a different formulation, ab79351.

    Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) (Negative control) cells labeling SOX2 with ab79351 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing showing no staining on NIH/3T3 cells. Counterstained with ab179504 anti-Tubulin (Rabbit mAb, 1/1000), ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution. Nuclear stained with DAPI.
    The negative controls are as follows:
    -ve control 1: ab79351 at 1/200 dilution, followed by ab150080 at 1/1000 dilution.
    -ve control 2: ab179504 at 1/1000 dilution, followed by ab150113 at 1/1000 dilution.

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