Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)
Key features and details
- Rabbit polyclonal to SNAIL + SLUG (phospho S246)
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-SNAIL + SLUG (phospho S246) antibody
See all SNAIL + SLUG primary antibodies -
Description
Rabbit polyclonal to SNAIL + SLUG (phospho S246) -
Host species
Rabbit -
Specificity
ab63568 detects endogenous levels of SNAIL only when phosphorylated at serine 246. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB HumanRecombinant fragment
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Immunogen
Synthesized phosphopeptide derived from human SNAIL around the phosphorylation site of serine 246 (T-F-SP-R-M).
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Positive control
- H29 nuclear lysate HuvEc cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution
Lane 1 : Extracts from HT29 cells (5-30µg), minus immunising peptide
Lane 2 : Extracts from HT29 cells (5-30µg) plus immunising peptide (5-10µg)
Predicted band size: 29 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
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Western blot - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilution
Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
Lane 2 : SLUG (SNAI2) Human Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Ab63568 recognizes the tagged recombinant SLUG and SNAIL proteins which have an expected molecular weight of 68 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG (phospho S246) antibody (ab63568)Immunofluorescent staining of HuvEc cells, using ab63568 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).
