Anti-SNAIL + SLUG antibody (ab180714)
Key features and details
- Rabbit polyclonal to SNAIL + SLUG
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-SNAIL + SLUG antibody
See all SNAIL + SLUG primary antibodies -
Description
Rabbit polyclonal to SNAIL + SLUG -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide within Human SNAIL+SLUG aa 236-264. The exact sequence is proprietary.
Database link: O95863 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SNAIL + SLUG antibody (ab180714)Anti-SNAIL + SLUG antibody (ab180714) at 1/500 dilution + Mouse heart tissue extracts
Predicted band size: 29 kDa
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Western blot - Anti-SNAIL + SLUG antibody (ab180714)All lanes : Anti-SNAIL + SLUG antibody (ab180714)
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Mouse lung tissue lysate
Lane 5 : Rat liver tissue lysate
Predicted band size: 29 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab180714)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SNAI1+SLUG with ab180714 at 1/50 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab180714)Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling SNAIL + SLUG with ab180714 at 1/100 dilution.
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Western blot - Anti-SNAIL + SLUG antibody (ab180714)Western blot analysis of extracts of various cell lines using ab180714 at the dilution 1/500.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab180714) Image is courtesy of an anonymous Abreviewab180714 staining SNAIL in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation in a basic buffer (TRIS+EDTA, pH9). Samples were incubated with primary antibody (1/500 in antibody diluent) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab180714)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 200X.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab180714)Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling SNAIL +SLUG with ab180714 at 1/100 dilution.
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Western blot - Anti-SNAIL + SLUG antibody (ab180714)Lane 1: Snail recombinant 0.1 ug (55 kDa)
Lane 2: Slug recombinant 0.1 ug (56 kDa)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab180714)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 400X.
