Anti-SNAIL + SLUG antibody (ab167609)
Key features and details
- Mouse polyclonal to SNAIL + SLUG
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-SNAIL + SLUG antibody
See all SNAIL + SLUG primary antibodies -
Description
Mouse polyclonal to SNAIL + SLUG -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB HumanRecombinant fragment
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Immunogen
Recombinant full length protein aa 1-264. Corresponding to Human SNAIL.
Sequence:MPRSFLVRKPSDPNRKPNYSELQDSNPEFTFQQPYDQAHLLAAIPPPEIL NPTASLPMLIWDSVLAPQAQPIAWASLRLQESPRVAELTSLSDEDSGKGS QPPSPPSPAPSSFSSTSVSSLEAEAYAAFPGLGQVPKQLAQLSEAKDLQA RKAFNCKYCNKEYLSLGALKMHIRSHTLPCVCGTCGKAFSRPWLLQGHVR THTGEKPFSCPHCSRAFADRSNLRAHLQTHSDVKKYQCQACARTFSRMSL LHKHQESGCSGCPR
Database link: NP_005976.2 -
Positive control
- SNAIl transfected 293T cell lysate. HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SNAIL + SLUG antibody (ab167609)All lanes : Anti-SNAIL + SLUG antibody (ab167609) at 1 µg/ml
Lane 1 : SNAIL transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat anti-Mouse IgG
Predicted band size: 29 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-SNAIL + SLUG antibody (ab167609)Immunofluorescent analysis of HeLa cells labeling SNAIL with ab167609 at 10 µg/ml. -
Western blot - Anti-SNAIL + SLUG antibody (ab167609)Lane 1 : Monoclonal Mouse anti-GST at 1 µg
Lanes 2 & 5 : Milk at 5 %
Lane 3 : Anti-SNAIL + SLUG antibody (ab167609) at 1 µg
Lane 4 : Monoclonal Mouse anti-GST at 1 µg/ml
Lane 6 : Anti-SNAIL + SLUG antibody (ab167609) at 1 µg/ml
Lanes 1-3 : SNAIL-GST fusion protein
Lanes 4-6 : SLUG-GST-fusion protein
Lysates/proteins at 0.2 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-mouse at 1/5000 dilution
Predicted band size: 29 kDa
Additional bands at: 55.5 kDa (possible tagged protein), 56.4 kDa (possible tagged protein)
Exposure time: 90 secondsSubstrate: Perkin Elmer
