Anti-SNAIL + SLUG antibody [20C8] (ab78105)
![Anti-SNAIL + SLUG antibody [20C8] (ab78105)](https://www.abcam.com/ps/products/78/ab78105/Images/ab78105-93900-anti-snail-slug-antibody-20c8-western-blot.jpg "Anti-SNAIL + SLUG antibody [20C8] (ab78105)")
Key features and details
- Mouse monoclonal [20C8] to SNAIL + SLUG
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-SNAIL + SLUG antibody [20C8]
See all SNAIL + SLUG primary antibodies -
Description
Mouse monoclonal [20C8] to SNAIL + SLUG -
Host species
Mouse -
Specificity
This antibody detects endogonous proteins in the following cell lines: MDAMB-453; MDAMB-435; MDAMB-231; A375. -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein (Human)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
20C8 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-SNAIL + SLUG antibody [20C8] (ab78105)All lanes : Anti-SNAIL + SLUG antibody [20C8] (ab78105) at 1/250 dilution
Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
Lane 2 : SLUG (SNAI2) Human Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Ab78105 recognizes the tagged recombinant SLUG and SNAIL proteins which have an expected molecular weight of 68 kDa -
![Sandwich ELISA - Anti-SNAIL + SLUG antibody [20C8] (ab78105)](https://www.abcam.com/ps/products/78/ab78105/Images/ab78105-93899-anti-snail-slug-antibody-20c8-sandwich-elisa.jpg)
Sandwich ELISA - Anti-SNAIL + SLUG antibody [20C8] (ab78105)Standard Curve for SNAIL ; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [20C8] to SNAIL + SLUG (ab78105) at 1µg/ml and Detector Antibody Rabbit polyclonal to SNAIL + SLUG - ChIP Grade (ab85931) at 0.5µg/ml.
