Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD133 antibody [EPR16508] (ab222782)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16508] to CD133
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-CD133 antibody [EPR16508]
See all CD133 primary antibodies
Description

Rabbit monoclonal [EPR16508] to CD133
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HAP1, Caco-2 (Human colorectal adenocarcinoma epithelial cell), NCCIT (Human pluripotent embryonic carcinoma epithelial cell) and HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate. Human fetal pancreas and fetal kidney lysate. IHC-P: Human pancreas and kidney tissue. Human bladder cancer tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16508
Isotype

IgG
Research areas

Western blot - Anti-CD133 antibody [EPR16508] (ab222782)

All lanes : Anti-CD133 antibody [EPR16508] (ab222782) at 1/2000 dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : PROM1 knockout HAP1 cell lysate

Lysates/proteins at 40 µg per lane.

Performed under reducing conditions.

Predicted band size: 97 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Exposure time: 8 minutes

ab222782 was shown to react with CD133 in wild-type HAP1 cells in western blot. Loss of signal was observed when PROM1 knockout sample was used. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab222782 overnight at 4°C at a 1 in 2000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with ECL reagent and imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR16508] (ab222782)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD133 with ab222782 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP)(ready use). Apical/endoluminal staining of human pancreatic ducts, and negative on Langerhans cells of the islets (PMID: 18261235). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)(ready to use).
Western blot - Anti-CD133 antibody [EPR16508] (ab222782)

All lanes : Anti-CD133 antibody [EPR16508] (ab222782) at 1/10000 dilution

Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 97 kDa

Exposure time: 5 seconds

Negative control: HeLa (PMID 9389721).

The MW observed is consistent with the literature (PMID 20717901; PMID 18645205).

Blocking buffer: 5% NFDM/TBST.
Western blot - Anti-CD133 antibody [EPR16508] (ab222782)

Anti-CD133 antibody [EPR16508] (ab222782) at 1/10000 dilution + NCCIT (Human pluripotent embryonic carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 97 kDa

Exposure time: 15 seconds

Blocking buffer: 5% NFDM/TBST.

The MW observed is consistent with the literature (PMID 20717901; PMID 18645205).
Western blot - Anti-CD133 antibody [EPR16508] (ab222782)

All lanes : Anti-CD133 antibody [EPR16508] (ab222782) at 1/2000 dilution

Lane 1 : Human fetal pancreas lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 97 kDa

Exposure time: 3 minutes

Blocking buffer: 5% NFDM/TBST.

The MW observed is consistent with the literature (PMID 20717901; PMID 18645205).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR16508] (ab222782)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD133 with ab222782 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ready to use). Membranous staining on parietal layer of Bowman’s capsule and apical staining on renal tubules in human kidney (PMID: 19092120). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ready to use).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [EPR16508] (ab222782)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling CD133 with ab222782 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ready to use). Membranous staining on human bladder cancer tumor cells (PMID: 22899341). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ready to use).
Anti-CD133 antibody [EPR16508] (ab222782)

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