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Alexa Fluor® 488 Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab195877)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [V9] to Vimentin - Cytoskeleton Marker
  • Suitable for: ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG1

Overview

  • Product name

    Alexa Fluor® 488 Anti-Vimentin antibody [V9] - Cytoskeleton Marker
    See all Vimentin primary antibodies

  • Description

    Alexa Fluor® 488 Mouse monoclonal [V9] to Vimentin - Cytoskeleton Marker

  • Host species

    Mouse

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Cat, Dog, Pig

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt: HeLa cells

  • General notes

    This monoclonal antibody to vimentin has been knockout validated in ICC/IF. The expected staining for vimentin was observed in wild type cells and no staining was seen in vimentin knockout cells.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab195877)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab195877)

    ab195877 staining Vimentin (shown in green) in wild-type HAP1 cells (top panel) and Vimentin knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195877 at 1/1000 dilution (shown in green) and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Alexa Fluor® 488 Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab195877)
    Flow Cytometry - Alexa Fluor® 488 Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab195877)

    Overlay histogram showing HeLa cells stained with ab195877 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195877, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was mouse IgG1 (monoclonal) Alexa Fluor® 488 (ab171463) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab195877)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab195877)

    ab195877 staining Vimentin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195877 at 1/1000 dilution (shown in green) and ab6046, Rabbit polyclonal to beta Tubulin at 1µg/ml overnight at +4°C. Ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed, was then incubated at 2µg/ml for 1h at room temperature (shown in red)  Nuclear DNA was labelled in blue with DAPI.

    This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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