Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] - BSA and Azide free (ab240087)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1394Y] to MHC class I + HLA A + HLA B - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] - BSA and Azide free
    See all MHC class I+HLA A+HLA B primary antibodies

  • Description

    Rabbit monoclonal [EPR1394Y] to MHC class I + HLA A + HLA B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Raji, THP-1, and U-937 whole cell lysates. Human liver tissue lysate; ICC/IF: Raji cells; IHC-P: Human colon carcinoma tissue; Flow Cyt: Raji cells.

  • General notes

    ab240087 is the carrier-free version of ab134189 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240087 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Flow Cytometry - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] - BSA and Azide free (ab240087)
    Flow Cytometry - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] - BSA and Azide free (ab240087)

    Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling MHC class I+HLA A+HLA B with Purified ab134189 at 1:100 dilution (10 µg/ml) (Red). Cells were fixed with / and permeabilised with /. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134189).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] - BSA and Azide free (ab240087)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] - BSA and Azide free (ab240087)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling MHC class I+HLA A+HLA B with purified ab134189 at 1/4000 dilution (0.26 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134189).

  • Western blot - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] - BSA and Azide free (ab240087)
    Western blot - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] - BSA and Azide free (ab240087)
    All lanes : Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189) at 1/2000 dilution (Purified)

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 2 : Human liver lysate
    Lane 3 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
    Lane 4 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 41 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.



    Blocking/Diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134189).

  • Immunocytochemistry/ Immunofluorescence - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] - BSA and Azide free (ab240087)
    Immunocytochemistry/ Immunofluorescence - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] - BSA and Azide free (ab240087)

    Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling MHC class I+HLA A+HLA B with purified ab134189 at 1/500 dilution (4.3 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134189).

  • Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] - BSA and Azide free (ab240087)
    Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] - BSA and Azide free (ab240087)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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