Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1394Y] to MHC class I + HLA A + HLA B
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y]
    See all MHC class I+HLA A+HLA B primary antibodies

  • Description

    Rabbit monoclonal [EPR1394Y] to MHC class I + HLA A + HLA B

  • Host species

    Rabbit

  • Specificity

    In our hands the MHC class I + HLA A + HLA B antibody (ab134189) did not work in WB with mouse samples, but customer data suggest that reactivity in ICC/IF and IHC. The target shows high sequence homology with HLA-C and HLA-E, and it is possible that this antibody will detect these proteins too.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MHC class I. The exact sequence is proprietary.

  • Positive control

    • WB: Raji, THP-1, and U-937 whole cell lysates. Human liver tissue lysate; ICC/IF: Raji cells; IHC-P: Human colon carcinoma tissue; Flow Cyt: Raji cells.

  • General notes

    We have preliminary internal testing data to indicate ab134189 may not react with rat. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR1394Y

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
    Flow Cytometry - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)

    Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells, labeling MHC class I+HLA A+HLA B with Purified ab134189 at 1:100 dilution (10 µg/ml) (Red). Cells were fixed with / and permeabilised with /. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling MHC class I+HLA A+HLA B with purified ab134189 at 1/4000 dilution (0.26 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
    Western blot - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
    All lanes : Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189) at 1/2000 dilution (Purified)

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 2 : Human liver lysate
    Lane 3 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
    Lane 4 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 41 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
    Immunocytochemistry/ Immunofluorescence - Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)

    Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling MHC class I+HLA A+HLA B with purified ab134189 at 1/500 dilution (4.3 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

  • Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)
    Anti-MHC class I + HLA A + HLA B antibody [EPR1394Y] (ab134189)

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