Antibodies, Proteins, Kits and Reagents for Life Science

345 091 ₸ Product size

100 µl 345 091 ₸

Order now and get it on Tuesday March 02, 2021

Anti-NADPH oxidase 4 antibody [UOTR1B493] (ab133303)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [UOTR1B493] to NADPH oxidase 4
Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
Reacts with: Rat, Human

Product name

Anti-NADPH oxidase 4 antibody [UOTR1B493]
See all NADPH oxidase 4 primary antibodies
Description

Rabbit monoclonal [UOTR1B493] to NADPH oxidase 4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Rat Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human NADPH oxidase 4. The exact sequence is proprietary.
Database link: Q9NPH5
(Peptide available as ab155071)
Positive control
- WB: Human fetal kidney tissue lysate, U87-MG, 293 and JAR cell lysates. IHC-P: Human and rat kidney tissues. ICC/IF: U87-MG cells. Flow Cyt: U87-MG
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

UOTR1B493
Isotype

IgG
Research areas

Western blot - Anti-NADPH oxidase 4 antibody [UOTR1B493] (ab133303)

All lanes : Anti-NADPH oxidase 4 antibody [UOTR1B493] (ab133303) at 1/1000 dilution (unpurified)

Lane 1 : Fetal kidney cell lysates
Lane 2 : U87-MG cell lysates
Lane 3 : 293 cell lysates
Lane 4 : JAR cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 67 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody [UOTR1B493] (ab133303)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling NADPH oxidase 4 with purified ab133303 at a dilution of 1/600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-NADPH oxidase 4 antibody [UOTR1B493] (ab133303)

Immunocytochemistry/Immunofluorescence analysis of U87-MG cells labelling NADPH with purified ab133303 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).
Flow Cytometry - Anti-NADPH oxidase 4 antibody [UOTR1B493] (ab133303)

Flow cytometry analysis of U87-MG (human glioblastoma) cells labeling with purified ab133303 at 1/230 dilution ( 10ug/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) )(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody [UOTR1B493] (ab133303) Lin et al Oncotarget. 2017 May 16; 8(20): 33586–33600. Published online 2017 Apr 4. doi: 10.18632/oncotarget.16829

Paraffin-embedded human normal colon and colorectal cancer tissue stained for NADPH oxidase 4 using ab133303 at 1/100 dilution in immunohistochemical analysis.

NOX4 expression in CRC samples compared with corresponding normal tissue. (a) Normal tissue; (b) CRC, scored as (−); (c) CRC, scored as (+); (d) CRC, scored as (++); (e) CRC, scored as (+++) (scale bar, 100 μm).

CRC = colorectal cancer.

From Figure 1D of Lin et al.

Lin et al Oncotarget. 2017 May 16; 8(20): 33586–33600. Published online 2017 Apr 4. doi: 10.18632/oncotarget.16829

Reproduced under the Creative Commons Attribution License (CC-BY).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody [UOTR1B493] (ab133303)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling NADPH oxidase 4 with purified ab133303 at a dilution of 1/600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-NADPH oxidase 4 antibody [UOTR1B493] (ab133303)

All lanes : Anti-NADPH oxidase 4 antibody [UOTR1B493] (ab133303) at 1/2000 dilution (purified)

Lane 1 : U87-MG whole cell lysate
Lane 2 : JAR whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 67 kDa
Observed band size: 67 kDa

Blocking and dilution buffer: 5% NFDM /TBST.
Anti-NADPH oxidase 4 antibody [UOTR1B493] (ab133303)

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