Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NM23A antibody [EPR3036] - BSA and Azide free (ab247543)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3036] to NM23A - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-NM23A antibody [EPR3036] - BSA and Azide free
    See all NM23A primary antibodies

  • Description

    Rabbit monoclonal [EPR3036] to NM23A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, MCF7 and Jurkat cell lysates.

  • General notes

    ab247543 is the carrier-free version of ab92327 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247543 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NM23A antibody [EPR3036] - BSA and Azide free (ab247543)
    Western blot - Anti-NM23A antibody [EPR3036] - BSA and Azide free (ab247543)
    All lanes : Anti-NM23A antibody [EPR3036] (ab92327) at 1/5000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : A375 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 17 kDa



    This data was developed using ab92327, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-NM23A antibody [EPR3036] - BSA and Azide free (ab247543)
    Flow Cytometry - Anti-NM23A antibody [EPR3036] - BSA and Azide free (ab247543)
    This data was developed using ab92327, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NM23A with unpurified ab92327 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NM23A antibody [EPR3036] - BSA and Azide free (ab247543)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NM23A antibody [EPR3036] - BSA and Azide free (ab247543)
    This data was developed using ab92327, the same antibody clone in a different buffer formulation.ab92327, at a dilution of 1/100, staining NM23A in formalin fixed, paraffin embedded Human lung adenocarcinoma tissue by Immunohistochemistry. Detection: DAB staining Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
  • Western blot - Anti-NM23A antibody [EPR3036] - BSA and Azide free (ab247543)
    Western blot - Anti-NM23A antibody [EPR3036] - BSA and Azide free (ab247543)
    All lanes : Anti-NM23A antibody [EPR3036] (ab92327) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : NME1 knockout HEK293T cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab92327).

    Lanes 1-4: Merged signal (red and green). Green - ab92327 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab92327 Anti-NM23A antibody [EPR3036] was shown to specifically react with NM23A in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266215 (knockout cell lysate ab258075) was used. Wild-type and NM23A knockout samples were subjected to SDS-PAGE. ab92327 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-NM23A antibody [EPR3036] - BSA and Azide free (ab247543)
    Anti-NM23A antibody [EPR3036] - BSA and Azide free (ab247543)

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