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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules

Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)

Price and availability

227 827 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP1334(2)Y] to alpha Tubulin (phospho Y272)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y]
    See all alpha Tubulin primary antibodies
  • Description

    Rabbit monoclonal [EP1334(2)Y] to alpha Tubulin (phospho Y272)
  • Host species

    Rabbit
  • Specificity

    This antibody detects alpha Tubulin phosphorylated at tyrosine 272.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A phospho synthetic peptide corresponding to residues surrounding tyrosine 272 of human alpha Tubulin.

  • Positive control

    • Jurkat whole cell lysate (ab7899)
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP1334(2)Y
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin

Images

  • Western blot - Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)
    Western blot - Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)
    All lanes : Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) at 1/50000 dilution

    Lane 1 : Jurkat cell lysates, untreated.
    Lane 2 : Jurkat cell lysates treated with pervanadate.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 54 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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