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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-NADPH oxidase 4 antibody (ab154244)

Price and availability

271 382 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-NADPH oxidase 4 antibody (ab154244)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to NADPH oxidase 4
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-NADPH oxidase 4 antibody
    See all NADPH oxidase 4 primary antibodies
  • Description

    Rabbit polyclonal to NADPH oxidase 4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Human NADPH oxidase 4 aa 252-564.
    Database link: Q9NPH5

  • Positive control

    • 293T, A431, Jurkat and Raji whole cell lysates; A431 cells; Human colon carcinoma tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Metabolism
    • Types of disease
    • Heart disease

Images

  • Western blot - Anti-NADPH oxidase 4 antibody (ab154244)
    Western blot - Anti-NADPH oxidase 4 antibody (ab154244)
    All lanes : Anti-NADPH oxidase 4 antibody (ab154244) at 1/1000 dilution

    Lane 1 : 293T whole cell extract
    Lane 2 : HeLa whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 67 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NADPH oxidase 4 antibody (ab154244)
    Immunocytochemistry/ Immunofluorescence - Anti-NADPH oxidase 4 antibody (ab154244)

    ab154244 staining NADPH oxidase 4 in A431 cells fixed in ice-cold MeOH for 5 minutes. Samples were incubated with primary antibody at 1:500 dilution. Hoechst 33342 staining was used (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody (ab154244)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody (ab154244)

    ab154244 staining  NADPH oxidase 4 in paraffin embedded rat kidney tissue by Immunohistochemistry  (IHC-P- paraffin embedded sections). Samples were incubated with primary antibody at 1:500 dilution.

  • Western blot - Anti-NADPH oxidase 4 antibody (ab154244)
    Western blot - Anti-NADPH oxidase 4 antibody (ab154244)
    Anti-NADPH oxidase 4 antibody (ab154244) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 67 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-NADPH oxidase 4 antibody (ab154244)
    Immunocytochemistry/ Immunofluorescence - Anti-NADPH oxidase 4 antibody (ab154244)
    Immunofluorescence analysis of methanol fixed A431 cells labeling NOX4 with ab154244 at 1/500. Lower panel shows costaining with Hoechst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody (ab154244)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NADPH oxidase 4 antibody (ab154244)
    Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling NOX4 with ab154244 at 1/250.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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