Anti-NADPH oxidase 4 antibody (ab154244)
Key features and details
- Rabbit polyclonal to NADPH oxidase 4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NADPH oxidase 4 antibody
See all NADPH oxidase 4 primary antibodies -
Description
Rabbit polyclonal to NADPH oxidase 4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human NADPH oxidase 4 aa 252-564.
Database link: Q9NPH5 -
Positive control
- 293T, A431, Jurkat and Raji whole cell lysates; A431 cells; Human colon carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-NADPH oxidase 4 antibody (ab154244) at 1/1000 dilution
Lane 1 : 293T whole cell extract
Lane 2 : HeLa whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 67 kDa
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ab154244 staining NADPH oxidase 4 in A431 cells fixed in ice-cold MeOH for 5 minutes. Samples were incubated with primary antibody at 1:500 dilution. Hoechst 33342 staining was used (Blue).
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ab154244 staining NADPH oxidase 4 in paraffin embedded rat kidney tissue by Immunohistochemistry (IHC-P- paraffin embedded sections). Samples were incubated with primary antibody at 1:500 dilution.
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Anti-NADPH oxidase 4 antibody (ab154244) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 67 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol fixed A431 cells labeling NOX4 with ab154244 at 1/500. Lower panel shows costaining with Hoechst 33342.
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Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling NOX4 with ab154244 at 1/250.