Anti-GAL4 antibody [EPR12710(B)] (ab175185)
![Anti-GAL4 antibody [EPR12710(B)] (ab175185)](https://www.abcam.com/ps/products/175/ab175185/Images/ab175185-201459-anti-gal4-antibody-epr12710b-western-blot.jpg "Anti-GAL4 antibody [EPR12710(B)] (ab175185)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12710(B)] to GAL4
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-GAL4 antibody [EPR12710(B)]
See all GAL4 primary antibodies -
Description
Rabbit monoclonal [EPR12710(B)] to GAL4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human GAL4 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P56470 -
Positive control
- Purchase matching WB positive control:Recombinant Human GAL4 protein
- HT-29, Human small intestine and rat small intestine lysates. Human colon and colon carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12710(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GAL4 antibody [EPR12710(B)] (ab175185)All lanes : Anti-GAL4 antibody [EPR12710(B)] (ab175185) at 1/1000 dilution
Lane 1 : HT-29 cell lysates
Lane 2 : Human small intestine lysates
Lane 3 : Rat small intestine lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 36 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (ab175185)](https://www.abcam.com/ps/products/175/ab175185/Images/ab175185-201456-anti-gal4-antibody-epr12710b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (ab175185)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling GAL4 with ab175185 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (ab175185)](https://www.abcam.com/ps/products/175/ab175185/Images/ab175185-201452-anti-gal4-antibody-epr12710b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (ab175185)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling GAL4 with ab175185 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-GAL4 antibody [EPR12710(B)] (ab175185)](https://www.abcam.com/ps/products/175/ab175185/Images/ab175185-4-benefits-of-recombinant-antibodies.png)
Anti-GAL4 antibody [EPR12710(B)] (ab175185)
