Anti-GAL4 antibody (ab229347)
Key features and details
- Rabbit polyclonal to GAL4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GAL4 antibody
See all GAL4 primary antibodies -
Description
Rabbit polyclonal to GAL4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human GAL4 (internal sequence). The exact sequence is proprietary.
Database link: P56470 -
Positive control
- WB: Rat and mouse colon extracts; HCT 116 whole cell extract; GAL4 transfected HEK-293T whole cell lysate. IHC-P: Human gastric cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody (ab229347)
Paraffin-embedded human gastric cancer tissue stained for GAL4 using ab229347 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-GAL4 antibody (ab229347)Anti-GAL4 antibody (ab229347) at 1/5000 dilution + Rat colon extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS-PAGE gel.
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Western blot - Anti-GAL4 antibody (ab229347)Anti-GAL4 antibody (ab229347) at 1/500 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS-PAGE gel.
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Western blot - Anti-GAL4 antibody (ab229347)Anti-GAL4 antibody (ab229347) at 1/1000 dilution + Mouse colon extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS-PAGE gel.
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Western blot - Anti-GAL4 antibody (ab229347)All lanes : Anti-GAL4 antibody (ab229347) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : GAL4-transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS-PAGE gel.
