Anti-CRISPR-Cas9 antibody (ab204448)
Key features and details
- Rabbit polyclonal to CRISPR-Cas9
- Suitable for: ICC/IF, IP, Flow Cyt, WB
- Reacts with: Streptococcus pyogenes
- Isotype: IgG
Overview
-
Product name
Anti-CRISPR-Cas9 antibody
See all CRISPR-Cas9 primary antibodies -
Description
Rabbit polyclonal to CRISPR-Cas9 -
Host species
Rabbit -
Specificity
This antibody reacts with CRISPR-Cas9, this antibody has been found to react with 6xHis and Flag tags. If using multiple tags we recommend ab189380.
-
Tested applications
Suitable for: ICC/IF, IP, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Streptococcus pyogenes -
Immunogen
Recombinant fragment corresponding to Streptococcus pyogenes CRISPR-Cas9 (N terminal). The immunogen was tagged with a 6xHis tag and an S tag this antibody has been found to react with 6xHis and Flag tags.
Database link: Q99ZW2 -
Positive control
- WB: HEK-293T cells transfected with DDDDK tag-CRISPR-Cas9 lysate. ICC/IF: U-2 OS cells transfected with DDDDK tag-CRISPR-Cas9. Flow Cyt: HEK-293T cells transfected with DDDDK tag-CRISPR-Cas9. IP: CRISPR-Cas9 IP in U-2 OS cells.
-
General notes
Older lots react with cas9 and the Flag and 6x His tags.
New lots react with only cas9 and the 6x His tag.
Please contact our scientific support team for clarification.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG
Images
-
Western blot - Anti-CRISPR-Cas9 antibody (ab204448)Lanes 1-2 : Anti-DDDDK tag antibody
Lanes 3-4 : Anti-CRISPR-Cas9 antibody (ab204448) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lanes 2 & 4 : HEK-293T cells transfected with DDDDK tag-CRISPR-Cas9 lysate
Lane 3 : HEK-293T cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 158 kDaWB: Transfer in nitrocelullose, block: 1 hour 5% milk in TBST (Tween= 0.2%),
Incubation with primary antibody o/n at 4°C in 5% milk TBST (Tween= 0.2%).
-
Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody (ab204448)Immunofluorescent analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells transfected with DDDDK tag-CRISPR-Cas9, labeling CRISPR-Cas9 with ab204448 at 1/10,000 dilution (green). Labeling with anti-DDDDK tag (red). Right hand panel shows merged image with DAPI staining (blue).
Protocol: Fixation with PFA 2% 10 min, permeabilization cold methanol, block with BSA 2% for 1h at RT, incubation with ab204448 1/10,000 1h 30 min at RT.
There is a low background staining with ab204448 which is minimized at 1/10000 dilution.
-
Flow Cytometry - Anti-CRISPR-Cas9 antibody (ab204448)Flow cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells (upper panel), and transfected with CRISPR-Cas9 (lower panel) labeling CRISPR-Cas9 with ab204448 at 1/5000 dilution.
Cells were fixed with 1% PFA and incubated with the primary antibody (1/5000) at RT for 2 hours.
-
Immunoprecipitation - Anti-CRISPR-Cas9 antibody (ab204448)Western blot analysis of immunoprecipitate using ab204448 in U-2 OS (Human bone osteosarcoma epithelial cell line) cells extracts.
WB: anti-DDDDK tag.
Lane 1: Input.
Lane 2: Immunoprecipitation with control serum (10µl).
Lane 3: Immunoprecipitation with ab204448 (10µl)
