Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C3 antibody [EPR2988] (ab181147)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2988] to C3
  • Suitable for: ICC, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-C3 antibody [EPR2988]
    See all C3 primary antibodies

  • Description

    Rabbit monoclonal [EPR2988] to C3

  • Host species

    Rabbit

  • Specificity

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: HepG2 cells; ICC: HepG2 cells; WB: HepG2 cell lysate. Human serum, plasma, and spinal cord lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-C3 antibody [EPR2988] (ab181147)
    Western blot - Anti-C3 antibody [EPR2988] (ab181147)
    All lanes : Anti-C3 antibody [EPR2988] (ab181147) at 1/1000 dilution (purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 2 : Human plasma lysate
    Lane 3 : Human serum lysate
    Lane 4 : Human spinal cord lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 187 kDa
    Observed band size: 115,34,40 kDa
    why is the actual band size different from the predicted?



    The molecular weights observed are consistent with what have been described in the literature (PMID: 23619360)

  • Immunocytochemistry - Anti-C3 antibody [EPR2988] (ab181147)
    Immunocytochemistry - Anti-C3 antibody [EPR2988] (ab181147)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling C3 with purified ab181147 at 1/250 dilution (0.4 µg/mL). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-C3 antibody [EPR2988] (ab181147)
    Flow Cytometry - Anti-C3 antibody [EPR2988] (ab181147)

    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling C3 with purified ab181147 at 1/20 dilution (5 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-C3 antibody [EPR2988] (ab181147)
    Anti-C3 antibody [EPR2988] (ab181147)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-C3 antibody [EPR2988] (ab181147)

© 2021 Astana Biomed Group LLP. All rights reserved.