Anti-C3 antibody (ab97462)
Key features and details
- Rabbit polyclonal to C3
- Suitable for: IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-C3 antibody
See all C3 primary antibodies -
Description
Rabbit polyclonal to C3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseHumanIP HumanWB Human
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1498 - 1625 of Human C3.
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Positive control
- WB: HepG2 and Huh7 whole cell lysate. ICC/IF: HeLa cells. IHC: H1299 xenograft tissue; mouse brain tissue. IP: HepG2 whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-C3 antibody (ab97462)All lanes : Anti-C3 antibody (ab97462) at 1/1000 dilution
Lane 1 : Huh7 whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 187 kDa5% SDS-PAGE.
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Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody (ab97462)HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling C3 with ab97462 at 1/200 dilution (green) in ICC/IF. Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes.
Nuclei were stained using Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 antibody (ab97462)Paraffin-embedded mouse brain tissue stained for C3 using ab97462 at 1/500 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-C3 antibody (ab97462)C3 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts using 5 μg of ab97462. Western blot was performed from the immunoprecipitate using ab97462.
Lane 1: HepG2 whole cell extract.
Lane 2: Control IgG instead of ab97462 in HepG2 whole cell extract.
Lane 3: ab97462 IP in HepG2 whole cell extract.
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Western blot - Anti-C3 antibody (ab97462)Anti-C3 antibody (ab97462) at 1/1500 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 187 kDa
7.5% SDS-PAGE. -
Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody (ab97462)ab97462 at 1/100 dilution staining C3 in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image shows cells co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 antibody (ab97462)ab97462 at 1/100 dilution staining C3 in H1299 xenograft by Immunohistochemistry, Paraffin-embedded tissue.
