Mouse sTNF RII ELISA Kit (TNFRSF1B) (ab202412)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse sTNF RII ELISA Kit (TNFRSF1B)
See all Soluble TNF Receptor II kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 5.5% Inter-assay Sample n Mean SD CV% Serum 3 8.6% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1 pg/ml -
Range
6.25 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 104 93% - 113% Serum 120 112% - 129% Cell culture media 100 97% - 106% EDTA Plasma 113 110% - 118% Cit plasma 114 100% - 125% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Horse -
Product overview
Mouse sTNF RII ELISA Kit (TNFRSF1B) (ab202412) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of sTNF RII (TNFRSF1B) protein in cit plasma, edta plasma, serum, urine, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse sTNF RII (TNFRSF1B) with 1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Tumor necrosis factor receptor superfamily member 1B (TNF RII) is a transmembrane protein with an extracellular domain that binds to TNF alpha. This extracellular domain can be proteolytically cleaved to make soluble TNF RII. The standard protein in this kit is soluble TNF RII and the capture and detector antibodies were raised against this region of TNF RII.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse sTNF RII Capture Antibody 1 x 600µl 10X Mouse sTNF RII Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BC 1 x 6ml Mouse sTNF RII Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
TNFRII (TNF Receptor II) is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF alpha but manifests a lower affinity to TNF beta. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNFRII consists of a 240 amino acid residue extracellular ligand binding portion, a 27 amino acid residue transmembrane segment and a 173 amino acid residue cytoplasmic domain. Soluble TNFRII (Soluble TNF Receptor II) is a 268 amino acid isoform containing the extracellular ligand-binding portion of TNFRII. It binds to TNF alpha and is capable of inhibiting TNF alpha induced activities by acting as a decoy receptor. -
Alternative names
- CD120b
- Etanercept
- p75
see all -
Database links
- Entrez Gene: 21938 Mouse
- SwissProt: P25119 Mouse
Images
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Other - Mouse sTNF RII ELISA Kit (TNFRSF1B) (ab202412)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse TNFRSF1B standard curve comparison data.Standard curve comparison between mouse TNFRSF1B SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 4-fold increase in sensitivity.
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Example of sTNF RII standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of sTNF RII in mouse serum, plasma (citrate), and plasma (EDTA).The concentrations of sTNF RII were measured in duplicate and interpolated from the sTNF RII standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 12 ng/mL in serum, 13.2 ng/mL in plasma (Citrate), and 12.8 ng/mL plasma (EDTA).
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Interpolated concentrations of sTNF RII in urine and RAW264.7 stimulated cell culture supernatant.The concentrations of sTNF RII were measured in duplicate and interpolated from the sTNF RII standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RII concentration was determined to be 13.3 ng/mL in urine and 3.9 ng/mL in RAW264.7 stimulated cell culture supernatant.
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Comparison of sTNF RII in unstimulated and PMA/PHA-stimulated RAW264.7, L929, and J774A.1 Cells.Cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. sTNF RII was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated RAW264.7, L929, an J774A.1 and cell culture media. Measured values were interpolated from the sTNF RII Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed. sTNF RII was undetectable in media.
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Sandwich ELISA - Mouse sTNF RII ELISA Kit (TNFRSF1B) (ab202412)To learn more about the advantages of recombinant antibodies see here.
