Mouse sTNF RII Matched Antibody Pair Kit (ab214482)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 7.81 pg/ml - 500 pg/ml
Overview
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Product name
Mouse sTNF RII Matched Antibody Pair Kit
See all Soluble TNF Receptor II kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
2.71 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse sTNF RII Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse sTNF RII.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab202412. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse sTNF RII Capture Antibody 2 x 50µg 1 x 50µg Mouse sTNF RII Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse sTNF RII Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- CD120b
- p75
- p75 TNF receptor
see all -
Database links
- Entrez Gene: 21938 Mouse
- SwissProt: P25119 Mouse
- Unigene: 235328 Mouse
Images
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Mouse sTNF RII standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse sTNF RII Matched Antibody Pair Kit (ab214482)To learn more about the advantages of recombinant antibodies see here.
