GTPase Assay Kit (ab270553)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
Overview
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Product name
GTPase Assay Kit -
Detection method
Colorimetric -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
GTPase Assay Kit (ab270553) employs a 96-well plate format with all of the reagents necessary for measuring GTPase activity. The kit also contains PiColorLock™ (a superior malachite green reagent) which has special additives to prevent backgrounds arising out of non-enzymatic GTP hydrolysis. The PiColorLock™ assay is based on the change in absorbance of the dye malachite green in the presence of phosphomolybdate complexes. Unlike most malachite dye-based solutions, PiColorLockTM gives a stable end- point signal and is not prone to precipitation. PiColorLockTM has special additives to prevent backgrounds arising from nonenzymatic GTP hydrolysis.
This product is manufactured by Expedeon, an Abcam company. It was previously called GTPase Enzyme Assay Kit. Expedeon product code 602-0120 is the same as the 2 x 96 tests size of this product.
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Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 2 x 96 tests ab274109 - 0.1 M Magnesium chloride 1 x 1500µl ab273991 - 0.1 mM Pi Standard 1 x 5ml ab274110 - 0.5 M Tris pH 7.5 1 x 5ml ab274107 - 96-well clear low binding plates 1 unit ab273989 - Accelerator 1 x 500µl ab274111 - GTP (lyophilized) 4 vials ab274293 - PiColorLock 1 x 10ml ab273990 - Stabilizer 1 x 5ml
Images
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Standard curve
Data provided for demonstration purposes only.
Standard Curve for GTPase Assay Kit (ab270553).
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Dynamic rangeDynamic Range for GTPase Assay Kit.
PiColorLockTM has been designed to have a large linear range, thus reducing the need for sample dilution. Competitors’ products are linear over a much narrower range of concentrations.