Human DR4 ELISA Kit (CD261) (ab46510)
Key features and details
- Sensitivity: 8 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-DR4 Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml DR4 Microplate (12 x 8 well strips) 1 unit 2 units DR4 Standard (lyophilized) Yellow 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
Research areas
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Relevance
Human Trail R1, CD261 or DR4 (Death Receptor 4) is a TNF receptor, which is a receptor for Trail (APO2 ligand). DR4 is involved in apoptosis. In the Trail receptor family, DR4, but also Trail R2 (DR5) transduce an apoptosis signal, whereas Trail R3 (DcR1) and Trail R4 (DcR2) antagonize TRAIL-induced apoptosis. Binding of trimeric Trail to DR4 induces apoptosis by oligomerization (likely to be trimerization) of the receptor. DR4 cDNA encodes a 468 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. DR4 shares 55% of its amino acid sequence with Trail R2. DR4 is widely expressed in normal tissue. -
Cellular localization
Cell Membrane; single-pass type I membrane protein -
Alternative names
- APO2
- CD261
- Death receptor 4
see all -
Database links
- Entrez Gene: 8797 Human
- SwissProt: O00220 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab46510