Anti-Myc tag antibody - ChIP Grade (ab9132)
Key features and details
- Goat polyclonal to Myc tag - ChIP Grade
- Suitable for: IP, ChIP, WB, ICC, ELISA
- Isotype: IgG
Overview
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Product name
Anti-Myc tag antibody - ChIP Grade
See all Myc tag primary antibodies -
Description
Goat polyclonal to Myc tag - ChIP Grade -
Host species
Goat -
Specificity
Antibody was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL /BSA). -
Tested applications
Suitable for: IP, ChIP, WB, ICC, ELISAmore details -
Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Myc tag antibody - ChIP Grade (ab9132)All lanes : Anti-Myc tag antibody - ChIP Grade (ab9132) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate at 0.2 µg
Lane 2 : E. coli whole cell lysate at 0.1 µg
Lane 3 : E. coli whole cell lysate at 0.05 µg
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ChIP - Anti-Myc tag antibody - ChIP Grade (ab9132)A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
