Anti-AKT3 + AKT1 antibody [E45] (ab32038)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E45] to AKT3 + AKT1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-AKT3 + AKT1 antibody [E45] -
Description
Rabbit monoclonal [E45] to AKT3 + AKT1 -
Host species
Rabbit -
Specificity
ab32038 detects Akt1 and Akt3. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human AKT1 aa 450-550.
Database link: P31749 -
Positive control
- Squamous lung carcinoma ICC/IF: SK-BR-3 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E45 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence analysis of SK-BR-3 (human breast adenocarcinoma epithelial cell) cells labeling AKT3+AKT1 with purified ab32038 at 1/500 dilution (2.1 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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All lanes : Anti-AKT3 + AKT1 antibody [E45] (ab32038) at 1/10000 dilution
Lane 1 : GFP tagged AKT1 transfected 293T cell lysate
Lanes 2 & 4 & 6 : Empty vector transfected 293T cell lysate
Lane 3 : GFP tagged AKT2 transfected 293T cell lysate
Lane 5 : GFP tagged AKT3 transfected 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Exposure time: 1 secondBlocking/Diluting buffer: 5% NFDM/TBST.
Observed band: 82kDa, 56kDa.
82kDa band represents GFP tagged AKT. 56kDa band represents endogenous AKT.
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