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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKB / AKT

Anti-AKT3 + AKT1 antibody [E45] (ab32038)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-AKT3 + AKT1 antibody [E45] (ab32038)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E45] to AKT3 + AKT1
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-AKT3 + AKT1 antibody [E45]
  • Description

    Rabbit monoclonal [E45] to AKT3 + AKT1
  • Host species

    Rabbit
  • Specificity

    ab32038 detects Akt1 and Akt3.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human AKT1 aa 450-550.
    Database link: P31749

  • Positive control

    • Squamous lung carcinoma ICC/IF: SK-BR-3 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E45
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKB / AKT
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • AKT
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 + AKT1 antibody [E45] (ab32038)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 + AKT1 antibody [E45] (ab32038)

    Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-AKT3 + AKT1 antibody [E45] (ab32038)
    Immunocytochemistry/ Immunofluorescence - Anti-AKT3 + AKT1 antibody [E45] (ab32038)

    Immunocytochemistry/ Immunofluorescence analysis of SK-BR-3 (human breast adenocarcinoma epithelial cell) cells labeling AKT3+AKT1 with purified ab32038 at 1/500 dilution (2.1 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Western blot - Anti-AKT3 + AKT1 antibody [E45] (ab32038)
    Western blot - Anti-AKT3 + AKT1 antibody [E45] (ab32038)
    All lanes : Anti-AKT3 + AKT1 antibody [E45] (ab32038) at 1/10000 dilution

    Lane 1 : GFP tagged AKT1 transfected 293T cell lysate
    Lanes 2 & 4 & 6 : Empty vector transfected 293T cell lysate
    Lane 3 : GFP tagged AKT2 transfected 293T cell lysate
    Lane 5 : GFP tagged AKT3 transfected 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Exposure time: 1 second


    Blocking/Diluting buffer: 5% NFDM/TBST.

    Observed band: 82kDa, 56kDa.

    82kDa band represents GFP tagged AKT. 56kDa band represents endogenous AKT. 

  • Anti-AKT3 + AKT1 antibody [E45] (ab32038)
    Anti-AKT3 + AKT1 antibody [E45] (ab32038)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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