Anti-AKT1 (mutated E17K) antibody [RM336] (ab238304)
![Anti-AKT1 (mutated E17K) antibody [RM336] (ab238304)](https://www.abcam.com/ps/products/238/ab238304/Images/ab238304-316097-2383041.png "Anti-AKT1 (mutated E17K) antibody [RM336] (ab238304)")
Key features and details
- Rabbit monoclonal [RM336] to AKT1 (mutated E17K)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AKT1 (mutated E17K) antibody [RM336]
See all AKT1 primary antibodies -
Description
Rabbit monoclonal [RM336] to AKT1 (mutated E17K) -
Host species
Rabbit -
Specificity
ab238304 reacts to the AKT E17K mutant. No cross reactivity with wild type AKT. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human AKT1. Corresponding to the AKT E17K mutant.
Database link: P31749 -
Positive control
- WB: HEK-293T transfected with a DNA construct encoding the AKT E17K mutant. IHC-P: HEK-293T cells transfected with a DNA construct encoding the AKT E17K mutation.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM336 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AKT1 antibody [RM336] (ab238304)Lanes 1-4 : Anti-AKT1 (mutated E17K) antibody [RM336] (ab238304) at 0.1 µg/ml
Lane 5 : Anti-AKT (clone RM252) rabbit monoclonal antibody at 0.1 µg/ml
Lane 1 : Cell lysate mixture of non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) and HEK-293T transfected with a DNA construct encoding the Akt E17K mutant; 92%:8% respectively
Lane 2 : Cell lysate mixture of non-transfected HEK-293T and HEK-293T transfected with a DNA construct encoding the Akt E17K mutant; 98%:2% respectively
Lane 3 : Cell lysate mixture of non-transfected HEK-293T and HEK-293T transfected with a DNA construct encoding the Akt E17K mutant; 99.5%:0.5% respectively
Lanes 4-5 : Cell lysate mixture of non-transfected HEK-293T and HEK-293T transfected with a DNA construct encoding the Akt E17K mutant; 100%:0% respectively
Predicted band size: 55 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 antibody [RM336] (ab238304)](https://www.abcam.com/ps/products/238/ab238304/Images/ab238304-316096-2383042.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 antibody [RM336] (ab238304)Formalin-fixed, paraffin-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with a DNA construct encoding either the AKT E17K mutation or Histone H3.3 stained for AKT with ab238304 at 0.2 µg/ml in immunohistochemical analysis.
Top panel: Transfected with AKT E17K mutation.
Bottom panel: Transfected with Histone H3.3.
