Anti-Myc tag antibody [9E10] (ab206486)
![Anti-Myc tag antibody [9E10] (ab206486)](https://www.abcam.com/ps/products/206/ab206486/Images/ab206486-356997-anti-myc-tag-antibody-9e10-westernblot-tagged-protein.png "Anti-Myc tag antibody [9E10] (ab206486)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [9E10] to Myc tag
- Suitable for: IHC-Fr, IHC-P, ICC/IF, IP, WB
Overview
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Product name
Anti-Myc tag antibody [9E10]
See all Myc tag primary antibodies -
Description
Rat monoclonal [9E10] to Myc tag -
Host species
Rat -
Specificity
This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICC/IF, IP, WBmore details -
Immunogen
Synthetic peptide corresponding to Human Myc tag aa 400 to the C-terminus (C terminal).
Database link: P01106 -
Epitope
Epitope located at aa 410-419; EQKLISEEDL -
General notes
ab206486 is a recombinant antibody that comprises the variable regions from the 9E10 hybridoma within a rat IgG1 scaffold.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
9E10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Myc tag antibody [9E10] (ab206486)All lanes : Anti-Myc tag antibody [9E10] (ab206486) at 2 µg/ml
Lane 1 : HEK-293 spiked with 5 ng multitag protein
Lane 2 : HEK-293 unspiked with 5 ng multitag protein
Lane 3 : 25 ng purified multitag protein
Lysates/proteins at 35 µg per lane.
Predicted band size: 48 kDa
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![Western blot - Anti-Myc tag antibody [9E10] (ab206486)](https://www.abcam.com/ps/products/206/ab206486/Images/ab206486-356996-anti-myc-tag-antibody-9e10-westernblot-tagged-protein-2.png)
Western blot - Anti-Myc tag antibody [9E10] (ab206486)All lanes : Anti-Myc tag antibody [9E10] (ab206486) at 1 µg/ml
Lane 1 : Multitag protein at 0.025 µg
Lane 2 : Multitag protein at 0.01 µg
Lane 3 : Multitag protein at 0.005 µg
Predicted band size: 48 kDa
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![Anti-Myc tag antibody [9E10] (ab206486)](https://www.abcam.com/ps/products/206/ab206486/Images/ab206486-3-benefits-of-recombinant-antibodies.png)
Anti-Myc tag antibody [9E10] (ab206486)
