Antibodies, Proteins, Kits and Reagents for Life Science

Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] (ab254406)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24159-81] to SS18-SSX1 Fusion Protein
Suitable for: Dot blot, IHC-P, Flow Cyt (Intra)
Reacts with: Human

Product name

Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81]
See all SS18-SSX1 Fusion Protein primary antibodies
Description

Rabbit monoclonal [EPR24159-81] to SS18-SSX1 Fusion Protein
Host species

Rabbit
Tested applications

Suitable for: Dot blot, IHC-P, Flow Cyt (Intra)more details
Unsuitable for: ICC,IP or WB
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Dot Blot: Human SS18 + SSX(1/2/4) fusion peptide. IHC-P: Human synovial sarcoma tissue. HEK-293T transfected with SSXT-SSX2 labeled 6X His tag. Flow Cyt: HEK-293T transfected with myc-tagged SSX18-SSX2 fusion construct. HEK-293T transfected with myc tagged SS18+SSX1+SSX2+SSX4 construct.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24159-81
Isotype

IgG

Dot Blot - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] (ab254406)

Dot Blot - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] (ab254406 used at a 1/1000 dilution (0.592 μg/ml)).

Lane 1: Human SS18 + SSX(1/2/4) fusion peptide

Lane 2: Human SS18 peptide

Lane 3: Human SSX(1/2/4) peptide

Blocking/Dilution buffer: 5% NFDM/TBST.

Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100000 dilution.

Exposure time is 3 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] (ab254406)

Immunohistochemical analysis of paraffin-embedded human synovial sarcoma tissue labeling SS18-SSX1 Fusion Protein with ab254406 at 1/5000 (0.118 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254406 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Nuclear staining on human synovial sarcoma (PMID: 32141887).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Flow Cytometry - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] (ab254406)

Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-tagged SSX18-SSX2 fusion construct (right panel) stained for SS18-SSX1 Fusion Protein using ab254406 at a 1/5000 dilution (0.01 µg), followed by Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution.

Isotype control (left panel) is Rabbit monoclonal IgG (ab172730).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] (ab254406)

Immunohistochemical analysis of paraffin-embedded (Panel A) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with SSXT-SSX2 labeled 6X His tag and (Panel B) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty plasmid. labeling SS18-SSX1 Fusion Protein with ab254406 at 1/5000 (0.118 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254406 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Nuclear staining on (A) HEK-293T transfected with SSXT-SSX2 labeled 6X His tag, no staining on (B) HEK-293T transfected with an empty plasmid.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] (ab254406)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling SS18-SSX1 Fusion Protein with ab254406 at 1/5000 (0.118 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254406 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: No staining on human kidney.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] (ab254406)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling SS18-SSX1 Fusion Protein with ab254406 at 1/5000 (0.118 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab254406 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Negative control: No staining on human stomach.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Flow Cytometry - Anti-SS18-SSX1 Fusion Protein antibody [EPR24159-81] (ab254406)

Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with myc tagged SS18+SSX1+SSX2+SSX4 construct (right panel) stained for SS18-SSX1 Fusion Protein using ab254406 at a 1/5000 dilution (0.01 µg), followed by Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1/2000 dilution.

Isotype control (left panel) is Rabbit monoclonal IgG (ab172730).
Anti-SS18 antibody [EPR24159-81] (ab254406)

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