Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ORP150 antibody [EP5891] (ab134944)

Key features and details

  • Rabbit monoclonal [EP5891] to ORP150
  • Suitable for: IP, ICC/IF, WB, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-ORP150 antibody [EP5891]
    See all ORP150 primary antibodies

  • Description

    Rabbit monoclonal [EP5891] to ORP150

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ORP150 aa 950-1050 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human colon and liver tissues; MCF7 cells; 293T, HeLa, HepG2, MCF7 and SH-SY5Y cell lysates IP: HeLa whole cell lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ORP150 antibody [EP5891] (ab134944)
    Western blot - Anti-ORP150 antibody [EP5891] (ab134944)
    All lanes : Anti-ORP150 antibody [EP5891] (ab134944) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 111 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EP5891] (ab134944)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EP5891] (ab134944)

    Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human colon tissue, labelling ORP150 using ab134944 at a 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-ORP150 antibody [EP5891] (ab134944)
    Flow Cytometry - Anti-ORP150 antibody [EP5891] (ab134944)

    Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling ORP150 with unpurified ab134944 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunoprecipitation - Anti-ORP150 antibody [EP5891] (ab134944)
    Immunoprecipitation - Anti-ORP150 antibody [EP5891] (ab134944)

    purifiedab134944 at 1/50 dilution (2µg) immunoprecipitating ORP150 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab134944 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134944 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/2000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 150 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ORP150 antibody [EP5891] (ab134944)
    Immunocytochemistry/ Immunofluorescence - Anti-ORP150 antibody [EP5891] (ab134944)
    Immunofluorescence staining of MCF7 cells, labelling ORP150 using ab134944 at a 1/250 dilution (red). Cell nuclei are shown in blue.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EP5891] (ab134944)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EP5891] (ab134944)

    Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human liver tissue, labelling ORP150 using ab134944 at a 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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