Anti-ORP150 antibody [6E3-2C3] (ab241049)
![Anti-ORP150 antibody [6E3-2C3] (ab241049)](https://www.abcam.com/ps/products/241/ab241049/Images/ab241049-319815-24104911.jpg "Anti-ORP150 antibody [6E3-2C3] (ab241049)")
Key features and details
- Mouse monoclonal [6E3-2C3] to ORP150
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-ORP150 antibody [6E3-2C3]
See all ORP150 primary antibodies -
Description
Mouse monoclonal [6E3-2C3] to ORP150 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Full length protein corresponding to Human ORP150. NP_006389.3
Database link: Q9Y4L1 -
Positive control
- WB: Rat liver lysate; HEK-293 cell lysate. ICC/IF: SK-N-BE cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
6E3-2C3 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ORP150 antibody [6E3-2C3] (ab241049)
Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (Human neuroblastoma cell line) cells labeling ORP150 using ab241049 at 1/100 dilution for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 minutes at RT.
Counterstains: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab241049 (D) Composite.
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![Western blot - Anti-ORP150 antibody [6E3-2C3] (ab241049)](https://www.abcam.com/ps/products/241/ab241049/Images/ab241049-319814-24104922.jpg)
Western blot - Anti-ORP150 antibody [6E3-2C3] (ab241049)Anti-ORP150 antibody [6E3-2C3] (ab241049) at 1/1000 dilution (16 hours at 4°C) + HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 20 µg
Secondary
Goat anti-Mouse IgG-HRP for 60 minutes at RT at 1/100 dilution
Developed using the ECL technique.
Predicted band size: 111 kDa
Exposure time: 6 minutesBlock: 2% BSA and 2% Skimmed milk in 1X TBST.
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![Western blot - Anti-ORP150 antibody [6E3-2C3] (ab241049)](https://www.abcam.com/ps/products/241/ab241049/Images/ab241049-319813-24104933.jpg)
Western blot - Anti-ORP150 antibody [6E3-2C3] (ab241049)Anti-ORP150 antibody [6E3-2C3] (ab241049) at 1/1000 dilution (16 hours at 4°C) + Rat liver lysate at 20 µg
Secondary
Goat anti-Mouse IgG-HRP for 60 minutes at RT at 1/100 dilution
Developed using the ECL technique.
Predicted band size: 111 kDa
Exposure time: 6 minutesBlock: 2% BSA and 2% Skimmed milk in 1X TBST.
