Antibodies, Proteins, Kits and Reagents for Life Science

Anti-ORP150 antibody [6E3-2C3] (ab241049)

Key features and details

Mouse monoclonal [6E3-2C3] to ORP150
Suitable for: WB, ICC/IF
Reacts with: Rat, Human
Isotype: IgG2b

Product name

Anti-ORP150 antibody [6E3-2C3]
See all ORP150 primary antibodies
Description

Mouse monoclonal [6E3-2C3] to ORP150
Host species

Mouse
Tested applications

Suitable for: WB, ICC/IFmore details
Species reactivity

Reacts with: Rat, Human
Immunogen

Full length protein corresponding to Human ORP150. NP_006389.3
Database link: Q9Y4L1
Positive control
- WB: Rat liver lysate; HEK-293 cell lysate. ICC/IF: SK-N-BE cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

6E3-2C3
Isotype

IgG2b
Light chain type

kappa
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-ORP150 antibody [6E3-2C3] (ab241049)

Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (Human neuroblastoma cell line) cells labeling ORP150 using ab241049 at 1/100 dilution for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.

Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 minutes at RT.

Counterstains: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain.

(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab241049 (D) Composite.
Western blot - Anti-ORP150 antibody [6E3-2C3] (ab241049)

Anti-ORP150 antibody [6E3-2C3] (ab241049) at 1/1000 dilution (16 hours at 4°C) + HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 20 µg

Secondary
Goat anti-Mouse IgG-HRP for 60 minutes at RT at 1/100 dilution

Developed using the ECL technique.

Predicted band size: 111 kDa

Exposure time: 6 minutes

Block: 2% BSA and 2% Skimmed milk in 1X TBST.
Western blot - Anti-ORP150 antibody [6E3-2C3] (ab241049)

Anti-ORP150 antibody [6E3-2C3] (ab241049) at 1/1000 dilution (16 hours at 4°C) + Rat liver lysate at 20 µg

Secondary
Goat anti-Mouse IgG-HRP for 60 minutes at RT at 1/100 dilution

Developed using the ECL technique.

Predicted band size: 111 kDa

Exposure time: 6 minutes

Block: 2% BSA and 2% Skimmed milk in 1X TBST.

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