Human APP (Amyloid Precursor Protein) knockout HEK-293T cell lysate (ab263777)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab255468 - Human APP knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human APP knockout HEK293T cell lysate (ab263777)
Lanes 1 and 5: HeLa cell lysate (20 µg)
Lanes 2 and 6: SH-SY5Y cell lysate (20 µg)
Lanes 3 and 7: Wild-type HEK-293T cell lysate (20 µg)
Lanes 4 and 8: APP knockout HEK-293T cell lysate (20 µg)
ab12266 was shown to react with Amyloid Precursor Protein in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255362 (knockout cell lysate ab263777) was used. Wild-type and Amyloid Precursor Protein knockout samples were subjected to SDS-PAGE. ab12266 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human APP knockout HEK293T cell lysate (ab263777)Homozygous: 1 bp insertion in exon 5