Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NEAS antibody [EPR3830-43] - BSA and Azide free (ab248472)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3830-43] to NEAS - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-NEAS antibody [EPR3830-43] - BSA and Azide free
    See all NEAS primary antibodies

  • Description

    Rabbit monoclonal [EPR3830-43] to NEAS - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248472 is the carrier-free version of ab133320 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248472 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NEAS antibody [EPR3830-43] - BSA and Azide free (ab248472)
    Western blot - Anti-NEAS antibody [EPR3830-43] - BSA and Azide free (ab248472)
    All lanes : Anti-NEAS antibody [EPR3830-43] (ab133320) at 1/10000 dilution

    Lane 1 : HeLa cell lysate + camptothecin
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate + staurosporine
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 285 kDa



    This data was developed using ab133320, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [EPR3830-43] - BSA and Azide free (ab248472)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [EPR3830-43] - BSA and Azide free (ab248472)
    This data was developed using ab133320, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Paraffin Embedded Human breast carcinoma tissue labelling NEAS with ab133320 at 1/500 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [EPR3830-43] - BSA and Azide free (ab248472)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [EPR3830-43] - BSA and Azide free (ab248472)
    This data was developed using ab133320, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Paraffin Embedded Human colon tissue labelling NEAS with ab133320 at 1/500 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-NEAS antibody [EPR3830-43] - BSA and Azide free (ab248472)
    Immunocytochemistry - Anti-NEAS antibody [EPR3830-43] - BSA and Azide free (ab248472)
    This data was developed using ab133320, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling NEAS with ab133320 at 1/250 dilution.
  • Anti-NEAS antibody [EPR3830-43] - BSA and Azide free (ab248472)
    Anti-NEAS antibody [EPR3830-43] - BSA and Azide free (ab248472)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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