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Neuroscience Cell Type Marker Neural Stem Cell marker

Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)

Price and availability

335 040 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1069Y] to Vimentin (phospho S38)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

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Overview

  • Product name

    Anti-Vimentin (phospho S38) antibody [EP1069Y]
    See all Vimentin primary antibodies
  • Description

    Rabbit monoclonal [EP1069Y] to Vimentin (phospho S38)
  • Host species

    Rabbit
  • Specificity

    ab52942 only detects Vimentin phosphorylated at Serine 38.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Vimentin aa 1-100 (phospho S38). The exact sequence is proprietary.
    Database link: P08670

  • Positive control

    • WB: HeLa cell lysate treated with Calyculin A.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 99% Tissue culture supernatant, 0.1% BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP1069Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Vimentin
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Images

  • Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
    Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
    All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/1000000 dilution

    Lane 1 : HeLa whole cell lysate - untreated
    Lane 2 : HeLa whole cell lysate - treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
    Dot Blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)

    Dot blot analysis of Vimentin (pS38) peptide (Lane 1) and Vimentin non-phospho peptide (Lane 2) labelling Vimentin (phospho S38) with ab52942 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
    Western blot - Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
    All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/200000 dilution

    Lane 1 : HeLa cell lysate untreated
    Lane 2 : HeLa cell lysate Calyculin A treated

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
    Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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