Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1069Y] to Vimentin (phospho S38)
- Suitable for: WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-Vimentin (phospho S38) antibody [EP1069Y]
See all Vimentin primary antibodies -
Description
Rabbit monoclonal [EP1069Y] to Vimentin (phospho S38) -
Host species
Rabbit -
Specificity
ab52942 only detects Vimentin phosphorylated at Serine 38. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide within Human Vimentin aa 1-100 (phospho S38). The exact sequence is proprietary.
Database link: P08670 -
Positive control
- WB: HeLa cell lysate treated with Calyculin A.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1069Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/1000000 dilution
Lane 1 : HeLa whole cell lysate - untreated
Lane 2 : HeLa whole cell lysate - treated with Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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Dot blot analysis of Vimentin (pS38) peptide (Lane 1) and Vimentin non-phospho peptide (Lane 2) labelling Vimentin (phospho S38) with ab52942 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/200000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate Calyculin A treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
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