Antibodies, Proteins, Kits and Reagents for Life Science

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100 µl 284 784 ₸ 10 µl 113 913 ₸

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Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944)

Key features and details

Rabbit monoclonal [EP1070Y] to Vimentin (phospho S72)
Suitable for: WB, IP, Dot blot
Reacts with: Mouse, Rat, Human
Isotype: IgG

Product name

Anti-Vimentin (phospho S72) antibody [EP1070Y]
See all Vimentin primary antibodies
Description

Rabbit monoclonal [EP1070Y] to Vimentin (phospho S72)
Host species

Rabbit
Tested applications

Suitable for: WB, IP, Dot blotmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Vimentin aa 50-150 (phospho ). The exact sequence is proprietary.
Positive control
- WB: HeLa; NIH/3T3; C6 lysates. IP: HeLa cells (untreated and Calyculin A treated).
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1070Y
Isotype

IgG
Research areas

Western blot - Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944)

All lanes : Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944) at 1/10000 dilution (purified)

Lane 1 : Untreated with C6 (Rat glial tumor glial cell) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) starved for 24 hours,then treated with 100ng Calyculin A for 60 minutes whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 54 kDa

Blocking and diluting buffer : 5% NFDM/TBST
Immunoprecipitation - Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944)

ab52944 (purified) at 1:20 dilution (2µg) immunoprecipitating Vimentin in HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate 10ug
Lane 2 (+): ab52944 & HeLa starved 24 hours, then treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52944 in HeLa starved 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysate
For western blotting, VeriBlot for IP Detection Reageant (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944)

All lanes : Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944) at 1/10000 dilution (purified)

Lane 1 : Untreated with HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) starved for 24 hours,then treated with 100nM Calyculin A for 30 minutes whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 54 kDa

Blocking and diluting buffer : 5% NFDM/TBST
Western blot - Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944)

All lanes : Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944) at 1/10000 dilution (purified)

Lane 1 : Untreated with NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100ng/ml Calyculin A for 30 minutes whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 54 kDa

Blocking and diluting buffer : 5% NFDM/TBST
Dot Blot - Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944)

Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated (1/2500 dilution)
Blocking & dilutiing buffer: 5% NFDM/TBST

Lane 1 sample: Vimentin (pS72) phospho peptide
Lane 2 sample: Vimentin non-phospho peptide

Exposure time: 3 minutes
Western blot - Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944)

All lanes : Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944) at 1/1000000 dilution

Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate treated with Calyculin A

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 54 kDa
Observed band size: 54 kDa

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