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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Human YY1 Antibody Pair - BSA and Azide free (ab253666)

Price and availability

670 ₸

Availability

Order now and get it on Wednesday March 24, 2021

Human YY1 Antibody Pair - BSA and Azide free (ab253666)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human YY1 Antibody Pair - BSA and Azide free
  • Assay type

    ELISA set
  • Range

    469 pg/ml - 3000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview


    • Human YY1 Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human YY1 in sandwich ELISAs and many other pair-based applications.

    • The pair can be used in variety of assays and platforms including but not limited to:

    •      - Sandwich ELISA

    •      - FRET/TR-FRET/HTR

    •      - Meso Scale Discovery® (MSD®)

    •      - Luminex® and bead-based assays

    •      - AlphaLISA®/AlphaScreen®

    •      - DELFIA® immunoassays

    •      - Simoa® and Single Molecule Counting (SMC™) immunoassays

    •      - Multiplex

    • Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

    •      - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.

    •      - Concentration of ~1 mg/ml as measured by the protein A280 method.

    • Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
    • We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

    • Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

    • Please note:

    • The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

    • The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

    • We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

    •  Antibody properties:

    •  Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

    •  Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

    •  Concentration: ~1 mg/ml

    •  Storage buffer: 100% PBS

    •  Form: Liquid

    •  Isotype: IgG

    •  Recombinant monoclonal antibodies offer several advantages including:

    •      - High batch-to-batch consistency and reproducibility

    •      - Improved sensitivity and specificity

    •      - Long-term security of supply

    •      - Animal-free production

    • For more information see here.

    • Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    • Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

    • Luminex is a trademark of Luminex Corporation, registered in the US and other countries.

    • AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

    • Simoa is a registered trademark of Quanterix, Inc.

    • SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components Identifier 10 x 96 tests
    Human YY1 Capture Antibody (unconjugated) —
    Human YY1 Detector Antibody (unconjugated) —
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
  • Function

    Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.
  • Sequence similarities

    Belongs to the YY transcription factor family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus matrix. Associated with the nuclear matrix.
  • Target information above from: UniProt accession P25490 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CF1
    • Delta
    • Delta transcription factor
    • INO80 complex subunit S
    • INO80S
    • NF E1
    • NF-E1
    • NFE1
    • OTTHUMP00000197459
    • Transcriptional repressor protein YY1
    • TYY1_HUMAN
    • UCR motif DNA binding protein
    • UCRBP
    • Yin and yang 1
    • Yin and Yang 1 protein
    • Yin Yang 1
    • Ying Yang 1
    • YY 1
    • YY 1 transcription factor
    • YY-1
    • YY1
    • YY1 transcription factor
    see all
  • Database links

    • Entrez Gene: 7528 Human
    • Omim: 600013 Human
    • SwissProt: P25490 Human
    • Unigene: 388927 Human

    Images

    • Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253666)
      Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253666)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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