Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 123 964 ₸

Order now and get it on Tuesday March 02, 2021

Anti-NeuroD1 antibody [EPR20766] (ab213725)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20766] to NeuroD1
Suitable for: WB, IHC-P, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-NeuroD1 antibody [EPR20766]
See all NeuroD1 primary antibodies
Description

Rabbit monoclonal [EPR20766] to NeuroD1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Y79 whole cell lysate; rat retina tissue lysate and mouse P3 (postnatal day 3) retina tissue lysate. IP: Y79 whole cell lysate. IHC-P: Mouse hippocampus tissue; rat hippocampus tissue. Flow cytometry: Y97 cells.
General notes
The Human species recommendation is based on the WB results. We do not guarantee IHC-P for Human.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20766
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (ab213725)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling NeuroD1 with ab213725 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on subgranular zone of the mouse hippocampus dentate gyrus (PMID: 19701197, PMID: 25825708) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (ab213725)

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling NeuroD1 with ab213725 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on subgranular zone of the rat hippocampus (PMID: 19701197, PMID: 25825708) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725)

All lanes : Anti-NeuroD1 antibody [EPR20766] (ab213725) at 1/1000 dilution

Lane 1 : Y79 (human retinoblastoma cell line) whole cell lysate
Lane 2 : Mouse P3 (postnatal day 3) retina lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 40 kDa
Observed band size: 39 kDa
why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST

Exposure times.

Lane 1: 10 seconds

Lane 2: 30 seconds
Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725)

Anti-NeuroD1 antibody [EPR20766] (ab213725) at 1000 cells + Rat retina tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 40 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/dilution buffer: 5% NFDM/TBST
Flow Cytometry - Anti-NeuroD1 antibody [EPR20766] (ab213725)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Y79 (human retinoblastoma cell line) cell line labeling NeuroD1 with ab213725 at 1/50 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] (ab213725)

NeuroD1 was immunoprecipitated from 0.35 mg of Y79 (human retinoblastoma cell line) whole cell lysate with ab213725 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213725 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution

Lane 1: Y79 whole cell lysate 10 μg (Input).
Lane 2: ab213725 IP in Y79 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213725 in Y79 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Anti-NeuroD1 antibody [EPR20766] (ab213725)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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