Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NeuroD1 antibody [EPR4008] (ab109224)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4008] to NeuroD1
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-NeuroD1 antibody [EPR4008]
    See all NeuroD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4008] to NeuroD1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Y79 and fetal brain lysates; ICC: Mouse primary neuron cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-NeuroD1 antibody [EPR4008] (ab109224)
    Western blot - Anti-NeuroD1 antibody [EPR4008] (ab109224)
    All lanes : Anti-NeuroD1 antibody [EPR4008] (ab109224) at 1/1000 dilution

    Lane 1 : Y79 lysates
    Lane 2 : Fetal brain lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 40 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry - Anti-NeuroD1 antibody [EPR4008] (ab109224)
    Immunocytochemistry - Anti-NeuroD1 antibody [EPR4008] (ab109224)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling NeuroD1 with ab109224 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in a little of mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

  • Anti-NeuroD1 antibody [EPR4008] (ab109224)
    Anti-NeuroD1 antibody [EPR4008] (ab109224)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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