Anti-NeuroD1 antibody [EPR17084] - BSA and Azide free (ab236148)
![Anti-NeuroD1 antibody [EPR17084] - BSA and Azide free (ab236148)](https://www.abcam.com/ps/products/236/ab236148/Images/ab236148-324771-anti-neurod1-antibody-epr17084-immunofluorescence.jpg "Anti-NeuroD1 antibody [EPR17084] - BSA and Azide free (ab236148)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17084] to NeuroD1 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Human
Overview
-
Product name
Anti-NeuroD1 antibody [EPR17084] - BSA and Azide free
See all NeuroD1 primary antibodies -
Description
Rabbit monoclonal [EPR17084] to NeuroD1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: SH-SY5Y cells.
-
General notes
Ab236148 is the carrier-free version of ab205300. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab236148 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17084 -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR17084] - BSA and Azide free (ab236148)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Y79 (Human retinoblastoma cell line) cells labeling NeuroD1 with ab205300 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic and nuclear staining on Y79 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab205300 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205300).
-
![Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR17084] - BSA and Azide free (ab236148)](https://www.abcam.com/ps/products/236/ab236148/Images/ab236148-311934-anti-neurod1-antibody-epr17084-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR17084] - BSA and Azide free (ab236148)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling NeuroD1 with ab205300 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic and nuclear staining on SH-SY5Y cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab205300 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205300).
-
![Anti-NeuroD1 antibody [EPR17084] - BSA and Azide free (ab236148)](https://www.abcam.com/ps/products/236/ab236148/Images/ab236148-3-benefits-of-recombinant-antibodies.png)
Anti-NeuroD1 antibody [EPR17084] - BSA and Azide free (ab236148)
