Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9524] to Growth Hormone - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free
    See all Growth Hormone primary antibodies

  • Description

    Rabbit monoclonal [EPR9524] to Growth Hormone - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human Growth Hormone. The exact sequence is proprietary.
    Database link: P01241

  • Positive control

    • Human Growth Hormone recombinant protein; Human pituitary lysate; Human pituitary tissue

  • General notes

    Ab183135 is the carrier-free version of ab155974. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab183135 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)
    Western blot - Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)
    Anti-Growth Hormone antibody [EPR9524] (ab155974) at 1/1000 dilution + Human pituitary lysate at 10 µg

    Secondary
    HRP labeled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 25 kDa
    Observed band size: 17-22 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab155974, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)
    This data was developed using ab155974, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pituitary tissue labeling Human Growth Hormone with ab155974 at 1/100 dilution Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)
    Western blot - Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)
    Anti-Growth Hormone antibody [EPR9524] (ab155974) at 1/1000 dilution + Human Growth Hormone recombinant protein with proprietary tag at 0.01 µg

    Secondary
    HRP labeled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 25 kDa
    Observed band size: 17-22 kDa why is the actual band size different from the predicted?



    This data was developed using ab155974, the same antibody clone in a different buffer formulation.

     

  • Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)
    Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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