Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)
![Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)](https://www.abcam.com/ps/products/183/ab183135/Images/ab183135-405660-anti-growth-hormone-antibody-epr9524-bsa-and-azide-free-western-blot.jpg "Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9524] to Growth Hormone - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free
See all Growth Hormone primary antibodies -
Description
Rabbit monoclonal [EPR9524] to Growth Hormone - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment within Human Growth Hormone. The exact sequence is proprietary.
Database link: P01241 -
Positive control
- Human Growth Hormone recombinant protein; Human pituitary lysate; Human pituitary tissue
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General notes
Ab183135 is the carrier-free version of ab155974. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab183135 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9524 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)Anti-Growth Hormone antibody [EPR9524] (ab155974) at 1/1000 dilution + Human pituitary lysate at 10 µg
Secondary
HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 17-22 kDa why is the actual band size different from the predicted?This data was developed using ab155974, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)](https://www.abcam.com/ps/products/183/ab183135/Images/ab183135-2-anti-growth-hormone-antibody-epr9524-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)This data was developed using ab155974, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pituitary tissue labeling Human Growth Hormone with ab155974 at 1/100 dilution Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Western blot - Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)](https://www.abcam.com/ps/products/183/ab183135/Images/ab183135-398908-anti-growth-hormone-antibody-epr9524-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)Anti-Growth Hormone antibody [EPR9524] (ab155974) at 1/1000 dilution + Human Growth Hormone recombinant protein with proprietary tag at 0.01 µg
Secondary
HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 17-22 kDa why is the actual band size different from the predicted?This data was developed using ab155974, the same antibody clone in a different buffer formulation.
-
![Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)](https://www.abcam.com/ps/products/183/ab183135/Images/ab183135-4-benefits-of-recombinant-antibodies.png)
Anti-Growth Hormone antibody [EPR9524] - BSA and Azide free (ab183135)
