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Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] - BSA and Azide free (ab272172)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23246-275] to Junctional Adhesion Molecule 1/JAM-A - BSA and Azide free
  • Suitable for: Flow Cyt, WB, ICC/IF, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] - BSA and Azide free
    See all Junctional Adhesion Molecule 1/JAM-A primary antibodies

  • Description

    Rabbit monoclonal [EPR23246-275] to Junctional Adhesion Molecule 1/JAM-A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: bEnd.3, Mouse testis, Mouse brain and RAW264.7, Recombinant Mouse JAM-A Fc Chimera Protein lysates. ICC/IF: bEnd.3 cell. Flow Cyt: Neuro-2a cell. IP: bEnd.3 cell.

  • General notes

    ab272172 is the carrier-free version of ab270446. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] - BSA and Azide free (ab272172)
    Immunocytochemistry/ Immunofluorescence - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] - BSA and Azide free (ab272172)
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized bEnd.3 cells labelling Junctional Adhesion Molecule 1/JAM-A with ab270446 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in bEnd.3 cell line ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270446).

  • Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] - BSA and Azide free (ab272172)
    Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] - BSA and Azide free (ab272172)
    Flow cytometric analysis of Neuro-2a (Mouse neuroblastoma neuroblast, Left) / bEnd.3 (Mouse brain endothelioma, Right) cells labelling Junctional Adhesion Molecule 1/JAM-A with ab270446 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270446).

  • Immunoprecipitation - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] - BSA and Azide free (ab272172)
    Immunoprecipitation - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] - BSA and Azide free (ab272172)

    Junctional Adhesion Molecule 1/JAM-A was immunoprecipitated from 0.35 mg bEnd.3 (mouse brain endothelioma), whole cell lysate with ab270446 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270446 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: bEnd.3 (mouse brain endothelioma), whole cell lysate 10 ug

    Lane 2: ab270446 IP in bEnd.3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270446 in bEnd.3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270446).

  • Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] - BSA and Azide free (ab272172)
    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] - BSA and Azide free (ab272172)

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