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Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1042Y] to Junctional Adhesion Molecule 1/JAM-A
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y]
    See all Junctional Adhesion Molecule 1/JAM-A primary antibodies

  • Description

    Rabbit monoclonal [EP1042Y] to Junctional Adhesion Molecule 1/JAM-A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Junctional Adhesion Molecule 1/JAM-A aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Hela cell lysate and human liver tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
    Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)

    Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
    Lane 2: Junctional Adhesion Molecule 1/JAM-A knockout HAP1 whole cell lysate (40 µg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab52647 observed at 32 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab52647 was shown to specifically react with Junctional Adhesion Molecule 1/JAM-A in wild-type HAP1 cells as signal was lost in Junctional Adhesion Molecule 1 knockout cells. Wild-type and Junctional Adhesion Molecule 1/JAM-A knockout samples were subjected to SDS-PAGE. ab52647 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder carcinoma tissue sections labeling Junctional Adhesion Molecule 1/JAM-A with Purified ab52647 at 1:100 dilution (6.15 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
    Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647) at 1/1000 dilution (purified) + TF-1 (Human Erythroleukemia erythroblast) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
    Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647) at 1/2000 dilution (unpurified) + Hela cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)

    Immunohistochemical staining of paraffin-embedded human liver using unpurified ab52647 at a 1/100 dilution.

  • Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)

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