Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free (ab269950)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23244-12] to Junctional Adhesion Molecule 1/JAM-A - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free
    See all Junctional Adhesion Molecule 1/JAM-A primary antibodies

  • Description

    Rabbit monoclonal [EPR23244-12] to Junctional Adhesion Molecule 1/JAM-A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human lung tissue lysate. HT-29, HUVEC and TF-1 whole cell lysate. ICC/IF: HT-29 cells. Flow Cyt: HT-29 cells, human B lymphocytes and human monocytes. IP: HT-29 whole cell lysate. Human lung tissue lysate.

  • General notes

    ab269950 is the carrier-free version of ab269948. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free (ab269950)
    Immunoprecipitation - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free (ab269950)

    Junctional Adhesion Molecule 1/JAM-A was immunoprecipitated from 0.35 mg human lung lysate 10µg with ab269948 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab269948 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: Human lung lysate 10µg.

    Lane 2: ab269948 IP in human lung lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269948 in human lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269948).

  • Immunoprecipitation - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free (ab269950)
    Immunoprecipitation - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free (ab269950)

    Junctional Adhesion Molecule 1/JAM-A was immunoprecipitated from 0.35 mg HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10µg with ab269948 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab269948 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: HT-29 whole cell lysate 10µg.

    Lane 2: ab269948 IP in HT-29 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269948 in HT-29 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269948).

  • Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free (ab269950)
    Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free (ab269950)

    Flow cytometric analysis of human B lymphocytes (Left) / human monocytes (Right) cells labeling Junctional Adhesion Molecule 1/JAM-A with ab269948 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    Human peripheral blood mononuclear cell (PBMC) co-stained with anti-CD19 conjugated to PE-Cy7 and anti-CD14 conjugated to BV510. JAM-A expression on B lymphocytes (CD19+) and monocytes (CD14+) population are shown respectively.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269948).

  • Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free (ab269950)
    Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free (ab269950)

    Flow cytometric analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling Junctional Adhesion Molecule 1/JAM-A with ab269948 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269948).

  • Immunocytochemistry/ Immunofluorescence - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free (ab269950)
    Immunocytochemistry/ Immunofluorescence - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free (ab269950)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma epithelial cell) cells labeling Junctional Adhesion Molecule 1/JAM-A with ab269948 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in HT-29 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269948).

  • Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free (ab269950)
    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23244-12] - BSA and Azide free (ab269950)

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