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Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] (ab267407)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [J10.4] to Junctional Adhesion Molecule 1/JAM-A
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4]
    See all Junctional Adhesion Molecule 1/JAM-A primary antibodies

  • Description

    Mouse monoclonal [J10.4] to Junctional Adhesion Molecule 1/JAM-A

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Human Junctional Adhesion Molecule 1/JAM-A.
    Database link: Q9Y624

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Caco-2, HT-29, A431 and HUVEC whole cell lysate. ICC/IF: HT-29 cells. Flow Cyt: HT-29 and Caco-2 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] (ab267407)
    Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] (ab267407)
    All lanes : Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] (ab267407) at 1.035 µg/ml

    Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
    Lane 4 : HUVEC (human umbilical vein endothelial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 32 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:10698320).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] (ab267407)
    Immunocytochemistry/ Immunofluorescence - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] (ab267407)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 cells labelling JAM-A with ab267407 at 20.7µg/ml, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in HT-29 cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab267407 at 20.7µg/ml followed by ab150080 at a 1/500 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

  • Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] (ab267407)
    Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] (ab267407)

    Flow cytometric analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labelling JAM-A with ab267407 at 1.035µg/ml (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] (ab267407)
    Flow Cytometry - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] (ab267407)

    Flow cytometric analysis of Caco-2 (Human colorectal adenocarcinoma epithelial cell) cells labelling JAM-A with ab267407 at 1.035µg/ml (Red) compared with a Mouse monoclonal IgG  (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] (ab267407)
    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [J10.4] (ab267407)

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