Antibodies, Proteins, Kits and Reagents for Life Science

Anti-VISTA antibody [BLR035F] (ab243891)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR035F] to VISTA
Suitable for: WB, ICC, IHC-P, IP
Reacts with: Human

Product name

Anti-VISTA antibody [BLR035F]
See all VISTA primary antibodies
Description

Rabbit monoclonal [BLR035F] to VISTA
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human VISTA aa 250-311 (C terminal). The exact sequence is proprietary. NP_071436.1 and Gene ID 64115.
Database link: Q9H7M9
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR035F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-VISTA antibody [BLR035F] (ab243891)

VISTA was immunoprecipitated from 1.0 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab243891 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243891 at 1/1000 dilution.
Lane 1: ab243891 IP in Jurkat whole cell lysate.

Lane 2: Contol IgG in Jurkat whole cell lysate.

Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot - Anti-VISTA antibody [BLR035F] (ab243891)

Western blot analysis using ab243891 at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate (50 µg).
Lane 2: MOLT-4 whole cell lysate (50 µg).
Lane 3: MJ whole cell lysate (50 µg).
Lane 4: RPMI 8226 whole cell lysate (50 µg).
Lane 5: HeLa whole cell lysate (50 µg).
Lane 6: KG-1 whole cell lysate (50 µg).
Lane 7: HEK-293T whole cell lysate (50 µg).
Lane 8: SUP-M2 whole cell lysate (50 µg).
Lane 9: MJ whole cell lysate (15 µg).

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [BLR035F] (ab243891)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for VISTA using ab243891 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunocytochemistry - Anti-VISTA antibody [BLR035F] (ab243891)

Formalin-fixed, paraffin-embedded NCI H226 cells labeling VISTA using ab243891 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Anti-VISTA antibody [BLR035F] (ab243891)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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