Anti-VISTA antibody [VISTA/3007] (ab257314)
Key features and details
- Mouse monoclonal [VISTA/3007] to VISTA
- Suitable for: IHC-P, Protein Array
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-VISTA antibody [VISTA/3007]
See all VISTA primary antibodies -
Description
Mouse monoclonal [VISTA/3007] to VISTA -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Protein Arraymore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human VISTA aa 1-350.
Database link: Q9H7M9 -
Positive control
- IHC-P: Human placenta and squamous cell carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
VISTA/3007 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Formalin-fixed, paraffin-embedded human placenta tissue stained for VISTA using ab257314 at 2 μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
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Formalin-fixed, paraffin-embedded human squamous cell carcinoma tissue stained for VISTA using ab257314 at 2 μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
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Protein array containing >19,000 full-length human proteins, probed with ab257314.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.